Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China; Department of Veterinary Anatomy & Histology, Shaheed Benazir Bhutto University of Veterinary and Animal Sciences, Sakrand, 67210, Sindh, Pakistan.
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Reprod Biol. 2020 Sep;20(3):441-446. doi: 10.1016/j.repbio.2020.07.006. Epub 2020 Jul 28.
In the present report, we determined the impact of dietary selenium (Se) deficiency and supplementation on the expression of two ER-resident selenoproteins i.e., Selenok and Selenom in the ovaries of aging mice. The mRNA expression of Selenok and Selenom (RT-qPCR) was significantly higher in the ovaries of mice fed diets supplemented with inorganic (ISe-S: 0.33 mg Se/kg) and organic (OSe-S: 0.33 mg Se/kg) Se compared to those fed a Se-deficient (Se-D: 0.08 mg Se/kg) diet and both Se-adequate (ISe-A: 0.15 mg Se/kg and OSe-A: 0.15 mg Se/kg) diets. Similarly, the protein signals of SELENOK (immunofluorescence assay) were also significantly higher in the Se-supplemented groups compared to those fed Se-D and Se-adequate (ISe-A and OSe-A) diets. Meanwhile, the rate of in vitro-produced blastocysts developing from MII oocytes was also evaluated and it was revealed that this rate was significantly higher in the Se-supplemented mice compared to those fed a Se-D diet. Altogether, the dietary Se supplementation increased the expression of Selenok (also its protein expression) and Selenom in the ovaries of aging mice, potentially contributing to an improved developmental potential of in vitro-matured M II oocytes.
在本报告中，我们确定了饮食硒（Se）缺乏和补充对衰老小鼠卵巢中两种内质网驻留硒蛋白即 Selenok 和 Selenom 表达的影响。与喂食缺乏 Se（Se-D：0.08mg Se/kg）饮食和两种 Se 充足（ISe-A：0.15mg Se/kg 和 OSe-A：0.15mg Se/kg）饮食的小鼠相比，喂食补充无机（ISe-S：0.33mg Se/kg）和有机（OSe-S：0.33mg Se/kg）Se 的饮食的小鼠的 Selenok 和 Selenom（RT-qPCR）mRNA 表达显著更高。同样，与喂食 Se-D 和 Se 充足（ISe-A 和 OSe-A）饮食的小鼠相比，补充 Se 的各组的 SELENOK 蛋白信号（免疫荧光测定）也显著更高。同时，还评估了从 MII 卵母细胞体外产生的囊胚的发育率，结果表明，与喂食 Se-D 饮食的小鼠相比，补充 Se 的小鼠的该率显著更高。总的来说，饮食 Se 补充增加了衰老小鼠卵巢中 Selenok（及其蛋白表达）和 Selenom 的表达，可能有助于提高体外成熟的 M II 卵母细胞的发育潜力。
