Juszczuk-Kubiak Edyta, Bujko Kamila, Cymer Monika, Wicińska Krystyna, Gabryszuk Mirosław, Pierzchała Mariusz
Laboratory of Genome and Transcriptome Sequencing, Department of Molecular Biology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzębiec, Poland.
Department of Animal Breeding, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzębiec, Poland.
Biol Trace Elem Res. 2016 Aug;172(2):336-345. doi: 10.1007/s12011-015-0592-0. Epub 2015 Dec 23.
Effect of selenium (Se) supplementation on the selenoprotein and lipid metabolism gene expression patterns in ruminants, especially in lambs is not yet fully understood. The aim of study was to evaluate the effect of Se supplementation on the messenger RNA (mRNA) expression patterns of selected selenoproteins and genes related to lipid metabolism in growing lambs. The experiment was conducted on 48 Polish Merino lambs divided into two groups (n = 24): control (C)-lambs fed with a basal diet (BD) with no Se supplementation, and supplemented (S)-lambs fed with a BD, supplemented with 0.5 mg Se/kg as sodium selenate for 8 weeks. Expression of 12 selenoproteins and six genes related to lipid metabolism was analyzed in the liver and longissimus dorsi (LD) muscle of growing lambs by qPCR. Significant differences were found in the expression of GPX1, GPX2, SEPM, SEPW1, SEP15, SEPGS2, and TXNRD1 in the liver, and GPX1, SEPP1, SEPN1, SEPW1, SEP15, and MSRB1 in the LD muscle between S and C lambs. Se supplementation mainly upregulated SEPW1, SEP15 (P < 0.001; P < 0.01) mRNA expression in the liver, and GPX1, SEPP1, SEPN1, SEPW1 (P < 0.001; P < 0.01) in the muscle of S group. On the other hand, significant decrease in GPX2 (P < 0.01), SEPM (P < 0.001), and SEPHS2 (P < 0.01) mRNA expression levels were observed in the liver of S group of lambs. Se supplementation did not affect PON1, LXRα, and PPARα mRNA expression levels, but a significant increase in mRNA levels of APOE and LPL in the LD muscle (P < 0.05) as well as LPL (P < 0.05) in the liver were noticed in the group of Se supplemented lambs. Our study confirmed that, in lambs, similarly to other species, mRNA expression patterns of several selenoproteins highly depend on dietary Se levels, and their expression is ruled by hierarchical principles and tissue-specific mechanisms. Moreover, the study showed that changes Se intake leads to different levels of genes expression related with lipid metabolism.
补充硒（Se）对反刍动物，尤其是羔羊体内硒蛋白和脂质代谢基因表达模式的影响尚未完全明确。本研究旨在评估补充硒对生长羔羊体内选定硒蛋白和脂质代谢相关基因的信使核糖核酸（mRNA）表达模式的影响。实验选用48只波兰美利奴羔羊，分为两组（每组n = 24只）：对照组（C），饲喂不添加硒的基础日粮（BD）；补硒组（S），饲喂添加了0.5毫克/千克亚硒酸钠的BD，持续8周。通过定量聚合酶链反应（qPCR）分析生长羔羊肝脏和背最长肌（LD）中12种硒蛋白和6种脂质代谢相关基因的表达情况。结果发现，补硒组（S）与对照组（C）羔羊相比，肝脏中谷胱甘肽过氧化物酶1（GPX1）、谷胱甘肽过氧化物酶2（GPX2）、硒蛋白M（SEPM）、硒蛋白W1（SEPW1）、硒蛋白15（SEP15）、硒代磷酸合成酶2（SEPGS2）和硫氧还蛋白还原酶1（TXNRD1），以及LD肌肉中GPX1、硒蛋白P1（SEPP1）、硒蛋白N1（SEPN1）、SEPW1、SEP15和甲硫氨酸亚砜还原酶B1（MSRB1）的表达存在显著差异。补硒主要上调了肝脏中SEPW1、SEP15（P < 0.001；P < 0.01）的mRNA表达，以及补硒组（S）肌肉中GPX1、SEPP1、SEPN1、SEPW1（P < 0.001；P < 0.01）的mRNA表达。另一方面，观察到补硒组（S）羔羊肝脏中GPX2（P < 0.01）、SEPM（P < 0.001）和硒蛋白H（SEPHS2）（P < 0.01）的mRNA表达水平显著下降。补硒对对氧磷酶1（PON1）、肝脏X受体α（LXRα）和过氧化物酶体增殖物激活受体α（PPARα）的mRNA表达水平没有影响，但补硒组羔羊LD肌肉中载脂蛋白E（APOE）和脂蛋白脂肪酶（LPL）的mRNA水平显著升高（P < 0.05），肝脏中LPL的mRNA水平也显著升高（P < 0.05）。我们的研究证实，与其他物种类似，羔羊体内几种硒蛋白的mRNA表达模式高度依赖于日粮硒水平，其表达受层级原则和组织特异性机制的调控。此外，研究表明，硒摄入量的变化会导致与脂质代谢相关的基因表达水平不同。
