Department of Food Science and Dietetics, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland.
Department of Basic Medical Sciences, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland.
Food Chem. 2021 Jan 15;335:127649. doi: 10.1016/j.foodchem.2020.127649. Epub 2020 Jul 24.
Rosa rugosa Thunb. seed oil (RR) extracted by supercritical CO was investigated. RR chemical composition, radical scavenging effect and oxidative stability were evaluated. RR aqueous emulsions were examined for cell cytotoxicity, proliferation, redox state and migration using mouse embryonic fibroblast Balb/3T3, human dermal fibroblast NHDF cell lines, and on neoplastic cell lines: acute monocytic leukemia THP-1 and lung adenocarcinoma A549. RR total contents of phytosterols, tocopherols, carotenoids and phenolics were 10115.23, 784.16, 40.32 and 10.30 mg/kg, respectively. Rich antioxidant composition of RR was reflected in its high antioxidant activity (2.1 mM/kg Trolox equivalent) as well as oxidative stability (activation energy 105.6 kJ/mol). The RR emulsions led to marked augmentation of the total cell protein content in BALB/3T3 and NHDF cultures, inhibited cancer cell migration and reduced ROS formation. The studied RR oil proved to have a remarkable combination of bioactive compounds and to exert an antioxidative and chemopreventive effects.
超临界 CO2 萃取的刺玫果籽油（RR）进行了研究。评估了 RR 的化学成分、自由基清除效果和氧化稳定性。使用小鼠胚胎成纤维细胞 Balb/3T3、人皮肤成纤维细胞 NHDF 细胞系以及肿瘤细胞系：急性单核细胞白血病 THP-1 和肺腺癌 A549，研究了 RR 水乳液的细胞毒性、增殖、氧化还原状态和迁移。RR 中植物甾醇、生育酚、类胡萝卜素和酚类物质的总含量分别为 10115.23、784.16、40.32 和 10.30mg/kg。RR 丰富的抗氧化成分反映在其高抗氧化活性（2.1mM/kg Trolox 当量）和氧化稳定性（活化能 105.6kJ/mol）上。RR 乳液导致 BALB/3T3 和 NHDF 培养物中总细胞蛋白含量显著增加，抑制癌细胞迁移并减少 ROS 形成。研究表明，RR 油具有显著的生物活性化合物组合，并具有抗氧化和化学预防作用。
