Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea.
School of Life Sciences, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea.
J Photochem Photobiol B. 2020 Sep;210:111959. doi: 10.1016/j.jphotobiol.2020.111959. Epub 2020 Jul 15.
Trigeminal ganglion (TG) neurons play an essential role in the sensory nerves of the face. Damaged TG neurons resulting from the accidental and non-intentional nerve lesions, commonly identified as neuropathic pain, which is known to cause intense pain and sensory abnormalities. For the treatment, surgical methods are conducted when the pharmacological treatment fails to provide satisfactory recovery. However, the process of surgery or drug intake can burden the patient or cause side effects. One of the logical choices of study becomes photobiomodulation (PBM) referred to as therapeutic approaches based on the interactions of visible or near-infrared (NIR) photons with biomolecules inside cells or tissues. In this study, we constructed a PBM illumination setup to stimulate the cultured primary TG neurons and compared the growth morphology between the non-irradiated control group and irradiation group with NIR laser of 808 nm wavelength. In addition, we applied various radiant exposures of 1, 2, and 10 J/cm with different pulse frequencies of 1, 10, and 100 Hz. We found that PBM could promote neurite growth of TG neurons, and it works at relatively low energy densities at 1 and 2 J/cm. The irradiation group in the pulsed wave mode with the frequency of 10 Hz was found to be the most effective when compared to other frequencies. Thus, PBM on TG neurons facilitated neuronal growth in vitro in a dose and frequency-dependent fashion. PBM may provide a potential therapeutic approach to treat damaged peripheral nerves.
三叉神经节（TG）神经元在面部感觉神经中起着至关重要的作用。由于意外和非故意的神经损伤而导致的 TG 神经元受损，通常被认为是神经性疼痛，这种疼痛会导致剧烈疼痛和感觉异常。对于治疗，当药物治疗无法提供满意的恢复时，会进行手术方法。然而，手术过程或药物摄入可能会给患者带来负担或引起副作用。研究的一个合理选择是光生物调节（PBM），它被称为基于可见光或近红外（NIR）光子与细胞或组织内生物分子相互作用的治疗方法。在这项研究中，我们构建了一个 PBM 照射装置来刺激培养的原代 TG 神经元，并比较了未经照射的对照组和用 808nm 波长的 NIR 激光照射组的生长形态。此外，我们应用了不同的辐照剂量 1、2 和 10J/cm，并分别采用 1、10 和 100Hz 的不同脉冲频率。我们发现 PBM 可以促进 TG 神经元的轴突生长，并且在 1 和 2J/cm 时以相对较低的能量密度起作用。与其他频率相比，在 10Hz 的脉冲波模式下的照射组被发现是最有效的。因此，PBM 以剂量和频率依赖的方式促进体外 TG 神经元的生长。PBM 可能为治疗受损周围神经提供一种潜在的治疗方法。
