犬特应性皮炎：木犀草素作为新型天然疗法的作用。
Canine atopic dermatitis: Role of luteolin as new natural treatment.
机构信息
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy.
Department of Health Sciences, University of Catanzaro "Magna Graecia", Catanzaro, Italy.
出版信息
Vet Med Sci. 2020 Nov;6(4):926-932. doi: 10.1002/vms3.325. Epub 2020 Aug 2.
BACKGROUND
Luteolin has been demonstrated to possess numerous biological effects. However, the effect of luteolin on LPS (Lipopolysaccharides) stimulation in CPEK cells has not been investigated.
HYPOTHESIS/OBJECTIVES: An in vitro model of atopic canine dermatitis was used to identify the antioxidant effect of luteolin as a new treatment that is capable of improving the conditions of veterinary patients.
METHODS
CPEK cells were treated with or without luteolin in the presence or absence of LPS. A cell viability assay was performed to test luteolin toxicity and the protective effect of luteolin after LPS stimulation. Additionally, enzyme-linked immunosorbent assay (ELISA) kits were used to detect the levels of IL-33, IL-1β, IL-6, and IL-8.
RESULTS
Luteolin was capable to significantly decrease levels expression of IL-33, IL 1β, IL-6, and IL-8.
CONCLUSIONS AND CLINICAL IMPORTANCE
Luteolin could be a new pharmacological treatment for canine atopic dermatitis.
背景
木犀草素具有多种生物学效应。然而，木犀草素对 LPS（脂多糖）刺激 CPEK 细胞的影响尚未得到研究。
假设/目的：本研究采用过敏性犬皮炎的体外模型，以确定木犀草素的抗氧化作用，作为一种新的治疗方法，能够改善兽医患者的病情。
方法
用或不用 LPS 处理 CPEK 细胞，并用木犀草素处理或不用木犀草素处理。进行细胞活力测定以测试 LPS 刺激后木犀草素的毒性和保护作用。此外，还使用酶联免疫吸附测定（ELISA）试剂盒检测 IL-33、IL-1β、IL-6 和 IL-8 的水平。
结果
木犀草素能够显著降低 IL-33、IL-1β、IL-6 和 IL-8 的表达水平。
结论和临床意义
木犀草素可能成为犬过敏性皮炎的一种新的药理治疗方法。
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