Yang Wan-Ju, Yan Jiang-Bo, Zhang Li, Zhao Fang, Mei Zhong-Ming, Yang Yan-Ning, Xiang Yi, Xing Yi-Qiao
Eye Center, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.
Department of Ophthalmology, The Central Hospital of Wuhan, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, Hubei 430014, P.R. China.
Exp Ther Med. 2020 Aug;20(2):901-909. doi: 10.3892/etm.2020.8751. Epub 2020 May 14.
Neonatal vascular ophthalmopathy is a refractory ophthalmologic disease, and is a major cause of blindness. Occurrence of neonatal vascular ophthalmopathy may be associated with Paxillin, a cellular adhesion molecule which promotes the migration of endothelial cells and angiogenesis. To explore the role of PXN in corneal angiogenesis, human umbilical vein endothelial cells were divided into five groups: i) Control group; ii) Empty vector-transfected control group; iii) PXN knockdown group (shPXN group); iv) PXN-negative control (NC) group; and v) PXN over-expressed group (overExp group). PXN protein levels, migration and tube formation were assessed in the different experimental groups. Mice were divided into four groups: i) Control; ii) Model; iii) shPXN; and iv) overExp groups. Tube formation was significantly increased in the overExp group compared with the empty vector-transfected control group (P<0.01). Tube formation was significantly decreased in the shPXN group compared with the PXN-NC group (P<0.01). In mice, blood corpuscles were significantly decreased in the shPXN group. PXN promoted the migration of endothelial cells and corneal angiogenesis. The results of the present study suggest a role for PXN in corneal angiogenesis and provide a theoretical basis and potential target for the treatment of corneal angiogenesis.
新生儿血管性眼病是一种难治性眼科疾病，是失明的主要原因。新生儿血管性眼病的发生可能与桩蛋白（Paxillin）有关，桩蛋白是一种细胞粘附分子，可促进内皮细胞迁移和血管生成。为了探究桩蛋白X（PXN）在角膜血管生成中的作用，将人脐静脉内皮细胞分为五组：i）对照组；ii）空载体转染对照组；iii）PXN敲低组（shPXN组）；iv）PXN阴性对照组（NC组）；v）PXN过表达组（overExp组）。评估不同实验组中的PXN蛋白水平、迁移和管腔形成情况。将小鼠分为四组：i）对照组；ii）模型组；iii）shPXN组；iv）overExp组。与空载体转染对照组相比，overExp组的管腔形成显著增加（P<0.01）。与PXN-NC组相比，shPXN组的管腔形成显著减少（P<0.01）。在小鼠中，shPXN组的血细胞显著减少。PXN促进内皮细胞迁移和角膜血管生成。本研究结果表明PXN在角膜血管生成中发挥作用，并为角膜血管生成的治疗提供了理论依据和潜在靶点。
