Wu Weiqi, Luo Hao, Wu Dan, Shi Menglin, Yu Jinhai, Liao Hongfei
Department of Ocular Oncology and Ocular Trauma, Affiliated Eye Hospital of Nanchang University, Jiangxi Research Institute of Ophthalmology and Visual Sciences, Key Laboratory of Ophthalmology of Jiangxi Province, Nanchang, Jiangxi 330006, P.R. China.
Department of Ophthalmology, The Third Affiliated Hospital of Nanchang University, Jiangxi Key Laboratory of Cancer Metastasis and Precision Treatment, Nanchang, Jiangxi 330008, P.R. China.
Exp Ther Med. 2022 Mar;23(3):227. doi: 10.3892/etm.2022.11152. Epub 2022 Jan 18.
The reduction in postoperative complications is a considerable concern after orbital reparation and reconstruction. Selecting the appropriate scaffold materials to improve the survival rates of the seeded cells is a challenge in tissue engineering. The aim of the present study was to evaluate the biological activity of a vascular endothelial cell-hydroxyapatite orbital complex, which was constructed with tissue engineering and used as an implant after enucleation of the eyeNew Zealand white rabbits were randomly divided into two groups that underwent hydroxyapatite orbital implant surgery in the right eye. The primary orbital microvascular endothelial cells were collected from the microvascular tissue and subsequently cultured. Then, hydroxyapatite ocular implants were cultured with vascular endothelial cells in the endothelial cell (EC) group, and implants were cultured without vascular endothelial cells in the blank group. Characterization of the cells was performed with immunofluorescence staining and a Transwell migration and cell tube formation assay. The levels of interleukin-8 (IL-8) and vascular endothelial growth factor (VEGF) in the rabbit conjunctiva were measured with an ELISA. The results showed that the levels of IL-8 were decreased in the EC group and increased in the blank group. The levels of VEGF were increased in the EC group when compared to the blank group with statistical significance. The average depth of the fibrovascular tissue was obviously thicker in the EC group compared with that found in the blank group. These findings suggest that the vascular endothelial cell-hydroxyapatite orbital implant complex may be an effective strategy with which to accelerate vascularization and reduce complications of infection with satisfactory biological activity.
眼眶修复与重建术后并发症的减少是一个相当令人关注的问题。在组织工程中，选择合适的支架材料以提高接种细胞的存活率是一项挑战。本研究的目的是评估血管内皮细胞 - 羟基磷灰石眼眶复合体的生物活性，该复合体通过组织工程构建，并在眼球摘除术后用作植入物。将新西兰白兔随机分为两组，对其右眼进行羟基磷灰石眼眶植入手术。从微血管组织中收集原代眼眶微血管内皮细胞，随后进行培养。然后，在内皮细胞（EC）组中，将羟基磷灰石眼植入物与血管内皮细胞一起培养，而在空白组中，植入物在没有血管内皮细胞的情况下培养。通过免疫荧光染色以及Transwell迁移和细胞管形成试验对细胞进行表征。用酶联免疫吸附测定法（ELISA）测量兔结膜中白细胞介素 - 8（IL - 8）和血管内皮生长因子（VEGF）的水平。结果显示，EC组中IL - 8水平降低，空白组中IL - 8水平升高。与空白组相比，EC组中VEGF水平升高，具有统计学意义。与空白组相比，EC组中纤维血管组织的平均深度明显更厚。这些发现表明，血管内皮细胞 - 羟基磷灰石眼眶植入复合体可能是一种有效的策略，可加速血管化并减少感染并发症，具有令人满意的生物活性。
