Wang Bangqin, Huang Xinxiang, Lin Jinying
Department of Rheumatology, The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China.
Exp Ther Med. 2020 Aug;20(2):910-916. doi: 10.3892/etm.2020.8779. Epub 2020 May 21.
Expression levels of serum cyclooxygenase (COX)-2 and forkhead box O3a (FOXO3a) in patients with rheumatoid arthritis (RA) and the correlation with disease activity were investigated. Sixty patients with RA admitted to the People's Hospital of Guangxi Zhuang Autonomous Region (study group; 28 active patients and 32 remissive patients), and further 30 healthy subjects undergoing physical examinations during the same period (control group) were enrolled in this study. RT-qPCR and enzyme-linked immunosorbent assay (ELISA) were used to detect the expression levels of COX-2 and FOXO3a in serum. According to DAS28 score, the patients were divided into active and remissive patients, between whom the expression levels were compared. Receiver operating characteristic (ROC) curves were plotted to analyze the diagnostic values of COX-2 and FOXO3a for disease activity. Pearson's correlation coefficient was used to analyze the correlation of the two markers with erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), and DAS28 score. The expression levels of COX-2 and FOXO3a in active and remissive patients were significantly higher than those in the control group (both P<0.05), and those in active patients were significantly higher than those in remissive patients (both P<0.05). The areas under the ROC curves (AUCs) of COX-2 and FOXO3a were 0.748 and 0.802, respectively, suggesting that the two markers have high diagnostic value. The expression levels of COX-2 and FOXO3a were positively correlated with ESR, CRP, and DAS28 score of active and remissive patients (both P<0.05). In conclusion, the expression levels of COX-2 and FOXO3a in patients with RA are upregulated, thus, the two markers may be involved in the development and progression of the disease. The expression levels of COX-2 and FOXO3a are related to the disease activity of RA, and therefore can be used as diagnostic indicators for the disease activity.
研究了类风湿关节炎（RA）患者血清环氧化酶（COX）-2和叉头框O3a（FOXO3a）的表达水平及其与疾病活动度的相关性。选取广西壮族自治区人民医院收治的60例RA患者（研究组，其中28例活动期患者和32例缓解期患者），并纳入同期进行体检的30名健康受试者（对照组）。采用逆转录定量聚合酶链反应（RT-qPCR）和酶联免疫吸附测定（ELISA）检测血清中COX-2和FOXO3a的表达水平。根据疾病活动度评分（DAS28）将患者分为活动期和缓解期，比较两组患者的表达水平。绘制受试者工作特征（ROC）曲线分析COX-2和FOXO3a对疾病活动度的诊断价值。采用Pearson相关系数分析这两个标志物与红细胞沉降率（ESR）、C反应蛋白（CRP）及DAS28评分的相关性。活动期和缓解期患者血清COX-2和FOXO3a的表达水平均显著高于对照组（均P<0.05），且活动期患者的表达水平显著高于缓解期患者（均P<0.05）。COX-2和FOXO3a的ROC曲线下面积（AUC）分别为0.748和0.802，提示这两个标志物具有较高的诊断价值。活动期和缓解期患者血清COX-2和FOXO3a的表达水平与ESR、CRP及DAS28评分均呈正相关（均P<0.05）。综上所述，RA患者血清COX-2和FOXO3a表达上调，二者可能参与了疾病的发生发展。COX-2和FOXO3a的表达水平与RA疾病活动度相关，可作为疾病活动度的诊断指标。
