Zhang Denglu, Du Xiaohang, Zhang Xufeng, Li Kailin, Kong Feng, Cheng Guanghui, Zhao Shengtian
Central Laboratory, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250014, P.R. China.
Key Laboratory for Kidney Regeneration of Shandong Province affiliated to The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.
Exp Ther Med. 2020 Aug;20(2):1307-1314. doi: 10.3892/etm.2020.8844. Epub 2020 Jun 5.
The shortage of transplantable organs impedes the development of tissue-engineered alternatives. Producing tissues similar to immature kidneys from pluripotent stem cells is possible , but the size of the organoids is limited. Furthermore, implantation is necessary for organoid development and functional maturation. In the present study, the induction procedure was optimized and kidney organoids derived from induced pluripotent stem cells were produced. The kidney organoids were examined by immunofluorescence and quantitative PCR. Then, a unilateral nephrectomy model was established that was beneficial to the compensatory proliferation of the other kidney. Finally, these organoids were implanted below the kidney capsules of immunodeficient mouse hosts that had been nephrectomized unilaterally. This implantation resulted in the enlargement of the organoids and the production of vascular cells. Although signs of organoid maturation were lacking in short-term culture , the present study provided a method for studying kidney organoid development .
可移植器官的短缺阻碍了组织工程替代物的发展。从多能干细胞中产生类似于未成熟肾脏的组织是可行的，但类器官的大小有限。此外，类器官的发育和功能成熟需要植入。在本研究中，优化了诱导程序，并产生了源自诱导多能干细胞的肾脏类器官。通过免疫荧光和定量PCR对肾脏类器官进行了检测。然后，建立了单侧肾切除模型，该模型有利于另一侧肾脏的代偿性增殖。最后，将这些类器官植入单侧肾切除的免疫缺陷小鼠宿主的肾包膜下。这种植入导致类器官增大并产生血管细胞。尽管在短期培养中缺乏类器官成熟的迹象，但本研究提供了一种研究肾脏类器官发育的方法。
