Verma Geetika, Sethi R S
College of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab India.
Lab Anim Res. 2020 Jul 29;36:22. doi: 10.1186/s42826-020-00055-z. eCollection 2020.
Ethion is an organophosphate used commonly in India despite being banned in many other countries. The present study was designed to study the interaction of ethion and lipopolysaccharide (LPS) together on lung after single low dose ethion exposure. Mice ( = 20) were alienated into control and treatment groups ( = 10 each). The treatment group was orally fed ethion (8 mg/kg/animal/day) dissolved in corn oil. The animals ( = 5 each) from both the groups were challenged with 80 μg lipopolysaccharide (LPS) intranasally and the remaining animals ( = 5 each) were administered normal saline solution after 24 h. Ethion along with LPS induced lung inflammation as indicated by increased neutrophils and total leukocyte count (TLC) in broncheoalveolar lavage fluid. Ethion induced histomorphological alterations in lung as shown by increased pulmonary inflammation score in histopathology. Real time PCR analysis showed that ethion followed by LPS resulted significant ( < 0.05) increase in pulmonary Toll-like receptor (TLR)-4 (48.53 fold), interleukin (IL)-1β (7.05 fold) and tumor necrosis factor (TNF)-α (5.74 fold) mRNA expression. LPS co-exposure suggested synergistic effect on TLR4 and TNF-α mRNA expression. Ethion alone or in combination with LPS resulted genotoxicity in blood cells as detected by comet assay. The data suggested single dietary ethion exposure alone or in conjunction with LPS causes lung inflammation and genotoxicity in blood cells.
乙硫磷是一种有机磷酸酯，尽管在许多其他国家已被禁用，但在印度仍被广泛使用。本研究旨在探讨单次低剂量乙硫磷暴露后，乙硫磷与脂多糖（LPS）共同作用于肺部的相互作用。将20只小鼠分为对照组和治疗组（每组10只）。治疗组经口灌胃给予溶解于玉米油中的乙硫磷（8毫克/千克/动物/天）。两组各5只动物经鼻内给予80微克脂多糖（LPS）进行攻击，其余动物（每组5只）在24小时后给予生理盐水。支气管肺泡灌洗液中中性粒细胞和总白细胞计数（TLC）增加，表明乙硫磷与LPS共同诱导了肺部炎症。组织病理学显示，乙硫磷诱导了肺部组织形态学改变，表现为肺部炎症评分增加。实时PCR分析表明，乙硫磷后再给予LPS导致肺部Toll样受体（TLR）-4（48.53倍）、白细胞介素（IL）-1β（7.05倍）和肿瘤坏死因子（TNF）-α（5.74倍）mRNA表达显著增加（P<0.05）。LPS共同暴露对TLR4和TNF-α mRNA表达有协同作用。彗星试验检测发现，单独使用乙硫磷或与LPS联合使用均可导致血细胞遗传毒性。数据表明，单独或与LPS联合进行单次饮食乙硫磷暴露会导致肺部炎症和血细胞遗传毒性。
