Sharma Prakriti, Sethi R S
Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India.
J Toxicol. 2024 Aug 29;2024:5261994. doi: 10.1155/2024/5261994. eCollection 2024.
Deltamethrin is an insecticide used to control harmful agricultural insects that otherwise damage crops and to control vector-borne diseases. Long-term exposure to deltamethrin results in the inflammation of the lungs. The present study elucidates the molecular mechanism underlying the deltamethrin-induced lung damage. The lung samples were extracted from the Swiss albino mice following the treatment of low (2.5 mg/kg) and high (5 mg/kg) doses of deltamethrin. The mRNA expression of TCR, IL-4, and IL-13 showed upregulation, while the expression of NFAT and FOS was downregulated following a low dose of deltamethrin. Moreover, the expression of TCR was downregulated with the exposure of a high dose of deltamethrin. Furthermore, the immunohistochemistry data confirmed the pattern of protein expression for TCR, FOS, IL-4, and IL-13 following a low dose of deltamethrin exposure. However, no change was seen in the TCR, NFAT, FOS, JUN, IL-4, and IL-13 immunopositive cells of the high-dose treatment group. Also, ELISA results showed increased expression of IL-13 in the BAL fluid of animals exposed to low doses of deltamethrin. Overall, the present study showed that deltamethrin exposure induces lung damage and immune dysregulation via dysregulating the NFAT signalling pathway.
溴氰菊酯是一种杀虫剂，用于控制有害农业昆虫（这些昆虫否则会损害农作物）以及控制媒介传播疾病。长期接触溴氰菊酯会导致肺部炎症。本研究阐明了溴氰菊酯诱导肺损伤的分子机制。在给瑞士白化小鼠分别注射低剂量（2.5毫克/千克）和高剂量（5毫克/千克）溴氰菊酯后，提取其肺样本。低剂量溴氰菊酯处理后，TCR、IL-4和IL-13的mRNA表达上调，而NFAT和FOS的表达下调。此外，高剂量溴氰菊酯暴露后，TCR的表达下调。此外，免疫组化数据证实了低剂量溴氰菊酯暴露后TCR、FOS、IL-4和IL-13的蛋白表达模式。然而，高剂量处理组的TCR、NFAT、FOS、JUN、IL-4和IL-13免疫阳性细胞未见变化。而且，ELISA结果显示，低剂量溴氰菊酯暴露动物的支气管肺泡灌洗（BAL）液中IL-13表达增加。总体而言，本研究表明，溴氰菊酯暴露通过失调NFAT信号通路诱导肺损伤和免疫失调。
