State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medcial University, Guiyang, PR China.
Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang, PR China.
Nat Prod Res. 2021 Dec;35(24):5883-5887. doi: 10.1080/14786419.2020.1800694. Epub 2020 Aug 3.
In this study, the crude polysaccharides of (CJP) was obtained from (Blume) Hook. f. et Thomson using hot water extraction method, which was separated and purified by DEAE-cellulose column and Sepharose CL-6B column. The structure of the purified component was preliminary characterized by gas chromatography-mass spectrometer (GC-MS), high performance gel permeation chromatography (HPGPC) and infrared spectroscopy (IR). By examining the degree of delayed-type hypersensitivity (DTH) in mice and carbon particle clearance index, the immunomodulatory activity was clarified. The results showed that the extraction rate of CJP was 24.9 ± 0.5%. After purification, the refined polysaccharides component (CJP-2) was obtained. The structural characterization results indicated that CJP-2 was mainly composed of mannose, glucose, and galactose, and its molecular weight was 790 Da. Immunomodulation results showed that the low and medium levels of CJP significantly enhanced the degree of DTH in mice (P < 0.05). CJP can improve the clearance index of mice and enhance their charcoal removal function. The study indicates that is a good source of polysaccharides, and CJP may be a new type of immunomodulator.
在这项研究中，采用热水提取法从（Blume）Hook. f. 及其 Thomson 中获得了（CJP）的粗多糖，并用 DEAE-纤维素柱和 Sepharose CL-6B 柱进行分离和纯化。通过气相色谱-质谱联用仪（GC-MS）、高效凝胶渗透色谱（HPGPC）和红外光谱（IR）对纯化成分的结构进行了初步表征。通过检测小鼠迟发型超敏反应（DTH）程度和碳粒清除指数，阐明了其免疫调节活性。结果表明，CJP 的提取率为 24.9±0.5%。经纯化后，得到精制多糖成分（CJP-2）。结构表征结果表明，CJP-2 主要由甘露糖、葡萄糖和半乳糖组成，分子量为 790 Da。免疫调节结果表明，低、中剂量的 CJP 显著增强了小鼠的 DTH 程度（P<0.05）。CJP 能提高小鼠的清除指数，增强其碳粒清除功能。研究表明，是一种很好的多糖来源，CJP 可能是一种新型免疫调节剂。
