College of Agriculture, Shanxi Agricultural University, Taiyuan 030031, China.
Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China.
Molecules. 2022 Aug 25;27(17):5454. doi: 10.3390/molecules27175454.
Polysaccharide is one of the main components of () and has good immune activity. However, the immune activity of polysaccharides with different molecular weights has not been systematically screened. In this study, the polysaccharides of from Pingshun of Shanxi Province (PSDSs) were first divided into two groups using ultrafiltration: 3.3 kDa (PSDSs-1) and more than 2000 kDa (PSDSs-2). The immunomodulatory effects of PSDSs with different molecular weights were evaluated in vitro and in vivo. In vitro experimental results showed that compared with Lipopolysaccharide-induced macrophages, PSDSs-1 increased TNF-α and IL-6 levels and decreased IL-10. Meanwhile, PSDSs-2 showed the opposite effect, indicating the difference in pro- and anti-inflammatory activities of PSDSs with different molecular weights. The immunosuppressive model of cyclophosphamide proved that PSDSs have immune-promoting function, with PSDSs-1 exhibiting a better effect than PSDSs-2. In vitro and in vivo experiments illustrated the complexity of PSDS immunomodulation. Further research on the functions of PSDs with different molecular weights is needed to lay a foundation for their classification and application.
多糖是（）的主要成分之一，具有良好的免疫活性。然而，不同分子量的多糖的免疫活性尚未得到系统筛选。在这项研究中，首先使用超滤将来自山西省平定的（PSDSs）多糖分为两组：3.3 kDa（PSDSs-1）和 2000 kDa 以上（PSDSs-2）。在体外和体内评估了不同分子量的 PSDSs 的免疫调节作用。体外实验结果表明，与脂多糖诱导的巨噬细胞相比，PSDSs-1 增加了 TNF-α 和 IL-6 水平，降低了 IL-10。同时，PSDSs-2 表现出相反的效果，表明不同分子量的 PSDSs 在促炎和抗炎活性方面存在差异。环磷酰胺的免疫抑制模型证明 PSDSs 具有免疫促进功能，PSDSs-1 的效果优于 PSDSs-2。体外和体内实验说明了 PSDS 免疫调节的复杂性。需要进一步研究不同分子量的 PSDs 的功能，为它们的分类和应用奠定基础。
