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小下游 ORF 的翻译增强了规范主要开放阅读框的翻译。

Translation of small downstream ORFs enhances translation of canonical main open reading frames.

机构信息

Stowers Institute for Medical Research, Kansas City, MO, USA.

Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS, USA.

出版信息

EMBO J. 2020 Sep 1;39(17):e104763. doi: 10.15252/embj.2020104763. Epub 2020 Aug 3.

DOI:10.15252/embj.2020104763
PMID:32744758
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7459409/
Abstract

In addition to canonical open reading frames (ORFs), thousands of translated small ORFs (containing less than 100 codons) have been identified in untranslated mRNA regions (UTRs) across eukaryotes. Small ORFs in 5' UTRs (upstream (u)ORFs) often repress translation of the canonical ORF within the same mRNA. However, the function of translated small ORFs in the 3' UTRs (downstream (d)ORFs) is unknown. Contrary to uORFs, we find that translation of dORFs enhances translation of their corresponding canonical ORFs. This translation stimulatory effect of dORFs depends on the number of dORFs, but not the length or peptide they encode. We propose that dORFs represent a new, strong, and universal translation regulatory mechanism in vertebrates.

摘要

除了规范的开放阅读框（ORF）之外，在真核生物的非翻译 mRNA 区域（UTRs）中还鉴定出了数千个翻译的小 ORF（包含少于 100 个密码子）。5'UTR 中的小 ORF（上游（u）ORF）通常会抑制同一 mRNA 中规范 ORF 的翻译。然而，3'UTR 中翻译的小 ORF（下游（d）ORF）的功能尚不清楚。与 uORF 相反，我们发现 dORF 的翻译会增强其相应规范 ORF 的翻译。dORF 的这种翻译刺激作用取决于 dORF 的数量，而不取决于它们编码的长度或肽。我们提出，dORF 代表了脊椎动物中一种新的、强大的、普遍的翻译调控机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eba8/7459409/5e0076f26ca8/EMBJ-39-e104763-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eba8/7459409/d64f56d85b78/EMBJ-39-e104763-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eba8/7459409/b4735fc3a448/EMBJ-39-e104763-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eba8/7459409/09dc3085ee57/EMBJ-39-e104763-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eba8/7459409/b5db736376ea/EMBJ-39-e104763-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eba8/7459409/dcf338976130/EMBJ-39-e104763-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eba8/7459409/fca183331d7b/EMBJ-39-e104763-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eba8/7459409/f6c6fc3cb206/EMBJ-39-e104763-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eba8/7459409/8e935ce7605e/EMBJ-39-e104763-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eba8/7459409/af04a7c16deb/EMBJ-39-e104763-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eba8/7459409/1637d171b667/EMBJ-39-e104763-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eba8/7459409/28a93d9cf65f/EMBJ-39-e104763-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eba8/7459409/5e0076f26ca8/EMBJ-39-e104763-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eba8/7459409/d64f56d85b78/EMBJ-39-e104763-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eba8/7459409/b4735fc3a448/EMBJ-39-e104763-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eba8/7459409/09dc3085ee57/EMBJ-39-e104763-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eba8/7459409/b5db736376ea/EMBJ-39-e104763-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eba8/7459409/dcf338976130/EMBJ-39-e104763-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eba8/7459409/fca183331d7b/EMBJ-39-e104763-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eba8/7459409/f6c6fc3cb206/EMBJ-39-e104763-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eba8/7459409/8e935ce7605e/EMBJ-39-e104763-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eba8/7459409/af04a7c16deb/EMBJ-39-e104763-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eba8/7459409/1637d171b667/EMBJ-39-e104763-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eba8/7459409/28a93d9cf65f/EMBJ-39-e104763-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eba8/7459409/5e0076f26ca8/EMBJ-39-e104763-g013.jpg

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