Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences. 33, bld. 2 Leninsky Ave., 119071, Moscow, Russia.
Gubkin University, Leninsky Prospect, 65/1, 119991, Moscow, Russia.
Int J Syst Evol Microbiol. 2020 Sep;70(9):4914-4919. doi: 10.1099/ijsem.0.004361.
A novel anaerobic, endospore-forming bacterium (strain M08 DMB) was isolated from a terrestrial mud volcano (Taman Peninsula, Russia). Cells of the strain were motile rods 1.3-2.0 µm long and 0.4 µm in diameter. The temperature range for growth was 5-42 °C, with an optimum at 30 °C. The pH range for growth was H 6.5-11.0, with an optimum at pH 8.0. Growth of strain M08 DMB was observed at NaCl concentrations of 0-5.0 % (w/v) with an optimum at 1.0 %. Strain M08 DMB utilized 3,4-dimethoxybenzoic acid, 2-methoxyphenol, carbon monoxide, glucose, fructose, mannose, xylose and yeast extract. The end product of glucose fermentation was acetate. The DNA G+C content of strain M08 DMB was 32.3 mol% (obtained via whole genome sequencing). The closest phylogenetic relative of strain M08 DMB was (family , class ) with 95.17 % 16S rRNA gene sequence similarity. Based on the phenotypic, genotypic and phylogenetic characteristics of the isolate, strain M08 DMB is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain of is M08 DMB (=KCTC 15840=VKM B-3387).
一种新型的厌氧、产芽孢细菌（菌株 M08 DMB）从陆地泥火山（俄罗斯塔曼半岛）中分离得到。该菌株的细胞为运动的杆状，长 1.3-2.0μm，直径 0.4μm。生长的温度范围为 5-42°C，最适温度为 30°C。生长的 pH 值范围为 H 6.5-11.0，最适 pH 值为 8.0。菌株 M08 DMB 在 0-5.0%（w/v）的 NaCl 浓度下生长，最适浓度为 1.0%。菌株 M08 DMB 可利用 3,4-二甲氧基苯甲酸、2-甲氧基苯酚、一氧化碳、葡萄糖、果糖、甘露糖、木糖和酵母提取物。葡萄糖发酵的终产物为乙酸。菌株 M08 DMB 的 DNA G+C 含量为 32.3mol%（通过全基因组测序获得）。菌株 M08 DMB 与 （科、纲）最为接近，16S rRNA 基因序列相似度为 95.17%。基于该分离株的表型、基因型和系统发育特征，菌株 M08 DMB 被认为代表了一个新的属种，命名为 sp. nov.。该菌株的模式菌株为 M08 DMB（=KCTC 15840=VKM B-3387）。
