Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Leninskiy Prospect, 33, Bld. 2, 119071, Moscow, Russia.
Gubkin University, Leninskiy Prospect, 65/1, 119991, Moscow, Russia.
Extremophiles. 2023 May 13;27(2):12. doi: 10.1007/s00792-023-01297-0.
A novel alkaliphilic sulfate-reducing bacterium, strain M08but, was isolated from a salsa lake of terrestrial mud volcano (Taman Peninsula, Russia). Cells were rod-shaped, motile and Gram-stain-negative. The temperature range for growth was 15-42 °C (optimum at 30 °C). The pH range for growth was 7.0-11.0, with an optimum at pH 8.5-9.0 Strain M08but used sulfate, thiosulfate, sulfite, dimethyl sulfoxide and arsenate as electron acceptors. Acetate, formate, butyrate, fumarate, succinate, glycerol and pyruvate were utilized as electron donors with sulfate. Fermentative growth was observed with fumarate, pyruvate, crotonate. Strain M08but grew chemolithoautotrophically with H and CO. The G + C content of the genomic DNA was 60.1%. The fatty acid profile of strain M08but was characterized by the presence of anteiso-C as the major component (68.8%). The closest phylogenetic relative of strain M08but was Desulfatitalea tepidiphila (the order Desulfobacterales) with 96.3% 16S rRNA gene sequence similarity. Based on the phenotypic, genotypic and phylogenetic characteristics of the isolate, strain M08but is considered to represent a novel species of the genus Desulfatitalea, with proposed name Desulfatitalea alkaliphila sp. nov. The type strain of Desulfatitalea alkaliphila is M08but (= KCTC 25382 = VKM B-3560 = DSM 113909 = JCM 39202 = UQM 41473).
一株新型嗜碱硫酸盐还原菌 M08but 从陆地泥火山的莎莎湖中分离得到（俄罗斯塔曼半岛）。细胞呈杆状，运动且革兰氏阴性。生长的温度范围为 15-42°C（最佳温度为 30°C）。生长的 pH 值范围为 7.0-11.0，最佳 pH 值为 8.5-9.0。M08but 菌株将硫酸盐、硫代硫酸盐、亚硫酸盐、二甲基亚砜和砷酸盐用作电子受体。乙酸盐、甲酸盐、丁酸盐、富马酸盐、琥珀酸盐、甘油和丙酮酸可用作硫酸盐的电子供体。观察到富马酸盐、丙酮酸、巴豆酸盐的发酵生长。M08but 菌株可以在 H 和 CO 存在下化能自养生长。基因组 DNA 的 G+C 含量为 60.1%。M08but 菌株的脂肪酸图谱的特征是存在为主成分的 anteiso-C（68.8%）。与 M08but 菌株最接近的亲缘关系是脱硫热脱硫杆菌（脱硫杆菌目），16S rRNA 基因序列相似性为 96.3%。基于分离株的表型、基因型和系统发育特征，M08but 菌株被认为代表脱硫脱硫杆菌属的一个新种，命名为脱硫脱硫杆菌碱亚种。脱硫脱硫杆菌碱亚种的模式菌株是 M08but（=KCTC 25382=VKM B-3560=DSM 113909=JCM 39202=UQM 41473）。
