Chai Li-Juan, Fang Guan-Yu, Xu Peng-Xiang, Zhang Xiao-Juan, Lu Zhen-Ming, Zhang Su-Yi, Wang Song-Tao, Shen Cai-Hong, Shi Jin-Song, Xu Zheng-Hong
Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, PR China.
National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, PR China.
Int J Syst Evol Microbiol. 2021 Sep;71(9). doi: 10.1099/ijsem.0.004991.
A Gram-stain-negative, coccus-shaped, obligately anaerobic, non-motile and non-spore-forming bacterium, designated strain JN500902, was isolated from the mud in a fermentation cellar used continuously over 30 years for Chinese strong-flavour production. Colonies were white, circular, convex and smooth-edged. Growth was observed at 20-40 °C (optimum, 37 °C), at pH 5.0-10 (optimum, pH 7.5), with 0-2 % (w/v) NaCl and with 0-4 % (v/v) ethanol. The Biolog assay demonstrated positive reactions of strain JN500902 in the metabolism of l-fucose and pyruvate. The predominant cellular fatty acids (>10 %) consisted of C and C. The major end metabolites of strain JN500902 were acetic acid and ethanol when incubated anaerobically in liquid reinforced clostridial medium. Acetate was the major organic acid end product. The complete genome size of strain JN500902 was 3 420 321 bp with 3327 identified genes. The G+C content was 43.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain JN500902 with the family , having low sequence similarity (92.8 %) to the nearest type strain, DSM 3224 and forming a clearly distinct branch. Core genome phylogenetic analysis of the isolate and 134 strains belonging to the family also revealed that strain JN500902 was well-separated from other genera of this family as a monophyletic clade. The average nucleotide identity and amino acid identity values between strain JN500902 and 134 strains were less than 74 and 65 %, respectively. Considering its polyphasic characteristics, strain JN500902 represents a novel genus and species within the family , for which the name gen. nov., sp. nov. is proposed. The type strain is JN500902 (=CICC 24502=JCM 33939).
从一个连续用于生产中国浓香型白酒30多年的发酵窖泥中分离出一株革兰氏染色阴性、球菌状、专性厌氧、不运动且不产芽孢的细菌，命名为菌株JN500902。菌落呈白色、圆形、凸起且边缘光滑。在20-40°C（最适温度为37°C）、pH 5.0-10（最适pH为7.5）、含0-2%（w/v）NaCl和0-4%（v/v）乙醇的条件下可观察到生长。Biolog分析表明菌株JN500902在L-岩藻糖和丙酮酸代谢中呈阳性反应。主要的细胞脂肪酸（>10%）由C和C组成。当在液体强化梭菌培养基中厌氧培养时，菌株JN500902的主要终产物是乙酸和乙醇。乙酸是主要的有机酸终产物。菌株JN500902的完整基因组大小为3420321 bp，共鉴定出3327个基因。G+C含量为43.5 mol%。基于16S rRNA基因序列的系统发育分析将菌株JN500902归为该科，与最接近的模式菌株DSM 3224的序列相似性较低（92.8%），并形成一个明显不同的分支。对该分离株和属于该科的134株菌株进行的核心基因组系统发育分析也表明，菌株JN500902作为一个单系分支与该科的其他属明显分开。菌株JN500902与134株菌株之间的平均核苷酸同一性和氨基酸同一性值分别小于74%和65%。考虑到其多相特征，菌株JN500902代表了该科内的一个新属和新种，为此提出新属名gen. nov.，新种名sp. nov.。模式菌株为JN500902（=CICC 24502=JCM 33939）。
