School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
Shenzhen Key Laboratory of Marine Microbiome Engineering, Institute for Advanced Study, Shenzhen University, Shenzhen, Guangdong, 518060, China.
Carbohydr Polym. 2020 Oct 15;246:116621. doi: 10.1016/j.carbpol.2020.116621. Epub 2020 Jun 17.
β-d-glucan is a natural non-digestible polysaccharide that can be selectively recognized by recognition receptors such as Dectin-1 receptors, resulting in an emerging interest on exploring its capacity for carrying biological information to desired organs or cells. CpG oligodeoxynucleotide (ODN) has the potentiality to initiate an immune-stimulatory cascade via activating B cells inducing proinflammatory cytokines, which is conducive to immunotherapy and nucleic acid vaccine. Herein, we developed a pH-sensitive delivery system loading with CpG ODN by introducing poly-ethylenimine (PEI) to a hyperbranched β-d-glucan (HBB) and coating with poly-ethylene glycol (PEG) shell via acidic liable Schiff bond. This delivery system exhibited a favorable biocompatibility and facilitated the cellular uptake of CpG ODN at pH 6.8 with the possibility of having higher accumulation in acidic cancer microenvironment. Furthermore, this carrier together with class B CpG ODN could enhance the secretion of cytokines including interleukin-6 and interferon-α as well as capable of interferon-α induction.
β-葡聚糖是一种天然的不可消化的多糖，它可以被 Dectin-1 受体等识别受体选择性识别，从而引起人们对探索其携带生物信息到所需器官或细胞的能力的兴趣。CpG 寡脱氧核苷酸（ODN）通过激活 B 细胞诱导促炎细胞因子，有可能引发免疫刺激级联反应，这有利于免疫治疗和核酸疫苗。在此，我们通过将聚亚乙基亚胺（PEI）引入超支化 β-葡聚糖（HBB）并通过酸性易缩醛 Schiff 键与聚乙二醇（PEG）壳进行涂层，开发了一种装载 CpG ODN 的 pH 敏感递送系统。该递送系统具有良好的生物相容性，并促进了 CpG ODN 在 pH 6.8 时的细胞摄取，有可能在酸性癌症微环境中具有更高的积累。此外，该载体与 B 类 CpG ODN 一起可以增强细胞因子（包括白细胞介素 6 和干扰素-α）的分泌，并能够诱导干扰素-α。
