Biofilm Formation by Gram-Negative Bacteria during Implant-Associated Infection.

Nonfermenting gram-negative bacteria and enterobacteria isolated from various biomaterials of patients of the orthopedic trauma hospital with implant-associated infection demonstrated significantly (p<0.05) higher biofilm formation capacity than the reference strains. The differences in biofilm formation kinetics were revealed: biofilm maturation and the beginning of its dispersion after 48-h incubation in enterobacteria and longer biofilm maturation phase in nonfermenting gram-negative bacteria. It was found that the strains isolated from sonication fluid have reliably (p<0.05) higher propensity to biofilm formation than bacteria isolated from aspirate and wound exudate.