Research Institute of Traumatology, Orthopedics and Neurosurgery, V. I. Razumovsky Saratov State Medical University, Ministry of Health of the Russian Federation, Saratov, Russia.
Bull Exp Biol Med. 2020 Jul;169(3):365-368. doi: 10.1007/s10517-020-04888-5. Epub 2020 Aug 3.
Nonfermenting gram-negative bacteria and enterobacteria isolated from various biomaterials of patients of the orthopedic trauma hospital with implant-associated infection demonstrated significantly (p<0.05) higher biofilm formation capacity than the reference strains. The differences in biofilm formation kinetics were revealed: biofilm maturation and the beginning of its dispersion after 48-h incubation in enterobacteria and longer biofilm maturation phase in nonfermenting gram-negative bacteria. It was found that the strains isolated from sonication fluid have reliably (p<0.05) higher propensity to biofilm formation than bacteria isolated from aspirate and wound exudate.
从骨科创伤医院植入物相关感染患者的各种生物材料中分离出的非发酵革兰氏阴性菌和肠杆菌表现出明显（p<0.05）更高的生物膜形成能力，比参考菌株更高。生物膜形成动力学的差异也被揭示：肠杆菌在孵育 48 小时后，生物膜成熟和开始分散的阶段，而非发酵革兰氏阴性菌的生物膜成熟阶段更长。研究发现，与从抽吸物和伤口渗出液中分离的细菌相比，从超声液中分离的菌株具有更高的生物膜形成倾向（p<0.05）。
