Xu Jianyi, Sheng Mengyao, Yang Zhou, Qiu Jiguo, Zhang Jun, Zhang Lei, He Jian
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China.
The College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China.
Curr Microbiol. 2020 Nov;77(11):3767-3772. doi: 10.1007/s00284-020-02145-6. Epub 2020 Aug 3.
A novel bacterial strain, designated HX-9-20, was isolated from the sediment collected from the wastewater outlet of an agricultural chemical plant in Maanshan city, Anhui province, PR China. Cells of strain HX-9-20 were Gram-staining-negative, rod-shaped, translucent, non-motile, and strictly aerobic. Growth was observed between 15 and 35 °C (optimum 30 °C), at pH 6.0-8.0 (optimum pH 7.0), and in the presence of 0-0.4% (w/v) NaCl (optimum 0.2%). The predominant cellular fatty acids were summed feature 3 (C ω7c and/or C ω6c), C and summed feature 8 (C ω6c and/or C ω7c). The major quinone was ubiquinone Q-8. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, and an aminophospholipid. Strain HX-9-20 contains 2-hydroxyputrescine and putrescine as the major polyamine. A phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HX-9-20 was affiliated with the genus Caenimonas, exhibiting the highest sequence similarities with Caenimonas koreensis EMB320 (97.3% similarity) and Ramlibacter humi 18×22-1 (97.0%), and less than 97.0% similarity with other type strains. The average nucleotide identity (ANI) and digital DNA-DNA hybridization values (dDDH) between HX-9-20 and C. koreensis EMB320 were 76.9% and 23.5% respectively. The ANI and dDDH between HX-9-20 and R. humi 18×22-1 were 80.3% and 23.6%, respectively. The G + C content of the genomic DNA was 67.5 mol%. On the basis of the polyphasic taxonomic data, strain HX-9-20 represents a novel species of the genus Caenimonas, for which the name Caenimonas sedimenti sp. nov. is proposed. The type strain is HX-9-20 (=KCTC 72473=CCTCC AB 2019266).
从中国安徽省马鞍山市一家农药厂废水排放口采集的沉积物中分离出一株新的细菌菌株，命名为HX-9-20。HX-9-20菌株的细胞革兰氏染色阴性，呈杆状，半透明，无运动性，严格需氧。在15至35°C（最适温度30°C）、pH 6.0 - 8.0（最适pH 7.0）以及0 - 0.4%（w/v）NaCl（最适0.2%）条件下可生长。主要的细胞脂肪酸为总特征3（C ω7c和/或C ω6c）、C以及总特征8（C ω6c和/或C ω7c）。主要醌类为泛醌Q-8。主要极性脂类为磷脂酰甘油、磷脂酰乙醇胺和一种氨基磷脂。HX-9-20菌株以2-羟基腐胺和腐胺作为主要多胺。基于16S rRNA基因序列的系统发育分析表明，HX-9-20菌株属于Caenimonas属，与韩国Caenimonas koreensis EMB320（相似性97.3%）和潮湿Ramlibacter humi 18×22-1（相似性97.0%）表现出最高的序列相似性，与其他模式菌株的相似性低于97.0%。HX-9-20与韩国Caenimonas koreensis EMB320之间的平均核苷酸同一性（ANI）和数字DNA-DNA杂交值（dDDH）分别为76.9%和23.5%。HX-9-20与潮湿Ramlibacter humi 18×22-1之间的ANI和dDDH分别为80.3%和23.6%。基因组DNA的G + C含量为67.5 mol%。基于多相分类学数据，HX-9-20菌株代表Caenimonas属的一个新物种，为此提出名为Caenimonas sedimenti sp. nov.的名称。模式菌株为HX-9-20（=KCTC 72473 = CCTCC AB 2019266）。
