Cell factory Research Centre, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea.
Department of Environmental Biotechnology, KRIBB School of Biotechnology, University of Science and Technology, Daejeon 34113, Republic of Korea.
J Microbiol Biotechnol. 2022 May 28;32(5):575-581. doi: 10.4014/jmb.2201.01023.
A Gram-stain-negative, white-coloured, and rod-shaped bacterium, strain DR4-4, was isolated from Daechung Reservoir, Republic of Korea, during bloom. Strain DR4-4 was most closely related to SGM1-15 and EMB320 with 98.1% 16S rRNA gene sequence similarities. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain DR4-4 and closely related type strains were below 79.46% and 22.30%, respectively. The genomic DNA G+C content was 67.5%. The major cellular fatty acids (≥10% of the total) were identified as C, cyclo C, summed feature 3 (C and/or C), and summed feature 8 (C and/or C). Strain DR4-4 possessed phosphatidylethanolamine, diphosphatidylglycerol, and phosphatidylglycerol as the main polar lipids and Q-8 as the respiratory quinone. The polyamine profile was composed of putrescine, cadaverine, and spermidine. The results of polyphasic characterization indicated that the isolated strain DR4-4 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is DR4-4 (=KCTC 82470 =JCM 34453).
一株革兰氏阴性、白色、杆状细菌，菌株 DR4-4，是从韩国大邱水库的水华期分离得到的。菌株 DR4-4 与 SGM1-15 和 EMB320 的 16S rRNA 基因序列相似度最高，为 98.1%。菌株 DR4-4 与密切相关的模式菌株的平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH）值分别低于 79.46%和 22.30%。基因组 DNA G+C 含量为 67.5%。主要的细胞脂肪酸（≥总脂肪酸的 10%）为 C、环 C、总和特征 3（C 和/或 C）和总和特征 8（C 和/或 C）。菌株 DR4-4 含有磷脂酰乙醇胺、双磷脂酰甘油和磷脂酰甘油作为主要极性脂类，以及 Q-8 作为呼吸醌。多胺图谱由腐胺、尸胺和亚精胺组成。多相特征分析的结果表明，分离到的菌株 DR4-4 代表了属内的一个新种，建议将其命名为 sp. nov.。模式菌株为 DR4-4（=KCTC 82470 =JCM 34453）。
