State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Microbial Culture Collection Center (GDMCC), Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.
College of Horticulture, South China Agricultural University, Guangzhou 510642, PR China.
Int J Syst Evol Microbiol. 2019 Nov;69(11):3460-3464. doi: 10.1099/ijsem.0.003641.
A Gram-stain-negative, aerobic and non-motile strain, designated 18x22-1, was isolated from a forest soil sample collected from Limushan Nature Reserve in Hainan Province, PR China. Growth occurred at 15-37 °C and pH 6.0-8.0 without NaCl. The 16S rRNA gene sequence analyses showed that strain 18x22-1 was closely related to DSM 14655 (98.5 %), followed by DSM 14656 (97.9 %) and other species and formed a stable cluster with DSM 14655, DSM 14656, JCM 19319 and s CCTCC AB 2015357. Results of chemotaxonomic analyses showed that ubiquinone-8 (Q-8) was the major respiratory quinone, and the major fatty acids (>10 % of the total amounts) were C and Ccyclo. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, two unidentified aminopholipids and four unidentified phospholipids. The draft genome sequence was 4.47 Mb long with a G+C content of 68.9 mol%. The average nucleotide identity and digital DNA-DNA hybridization values between strain 18x22-1 and four closely related type strains were in the range of 79.3-82.3 % and 21.9-25.1 %, respectively. The results of phenotypic, phylogenetic and chemotaxonomic analyses supported that strain 18x22-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 18x22-1 (=GDMCC 1.1584=KCTC 52922).
一株革兰氏阴性、需氧、不运动的菌株，命名为 18x22-1，从中国海南省黎母山自然保护区的森林土壤样本中分离得到。该菌株在 15-37℃、pH6.0-8.0、无 NaCl 的条件下生长。16S rRNA 基因序列分析表明，菌株 18x22-1与 DSM 14655（98.5%）密切相关，其次是 DSM 14656（97.9%）和其他种，与 DSM 14655、DSM 14656、JCM 19319 和 s CCTCC AB 2015357 形成稳定的聚类。化学分类分析结果表明，醌-8（Q-8）为主要呼吸醌，主要脂肪酸（占总量的>10%）为 C 和 Ccyclo。主要极性脂为磷脂酰甘油、双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、两种未鉴定的胺磷脂和四种未鉴定的磷脂。基因组草图长 4.47 Mb，G+C 含量为 68.9 mol%。菌株 18x22-1 与 4 株近缘模式菌株的平均核苷酸同一性和数字 DNA-DNA 杂交值分别在 79.3-82.3%和 21.9-25.1%之间。表型、系统发育和化学分类分析的结果支持菌株 18x22-1 代表一个新的属种，提议将其命名为 sp. nov.。模式菌株为 18x22-1（=GDMCC 1.1584=KCTC 52922）。
