The World Egg Bank, Phoenix, AZ, 85020, USA.
Ovation Fertility, Newport Beach, CA, 92663, USA.
J Assist Reprod Genet. 2020 Oct;37(10):2393-2398. doi: 10.1007/s10815-020-01904-5. Epub 2020 Aug 3.
Examine good tissue practices as relates to in vitro fertilization, biopsying, and vitrificationto compare current knowledge of ova, sperm, and embryos as vectors for disease transmission as it relates to our current knowledge regarding the SARS-CoV-2 virus.Unknown risks relating to the SARS-CoV-2 virus and sperm, ova, and embryos necessitate a reexamining of how human IVF is performed. Over the last decade, improvements in cryosurvival and live birth outcomes have been associated with zona pellucida breaching procedures (e.g., blastocyst collapsing and biopsying). In turn, today embryos are generally no longer protected by an intact zona pellucida when vitrified and in cryostorage. Additionally, high security storage containers have proven to be resilient to potential cross-contamination and reliable for routine human sperm freezing and embryo vitrification.Several options to current IVF practices are presented that can effectively mitigate the risks of cross-contamination and infection due to the current Covid-19 pandemic or other viral exposures. The question remains; is heightened security and change warranted where the risks of disease transmission likely remain negligible?
检查与体外受精、活检和玻璃化相关的良好组织实践，比较目前关于卵母细胞、精子和胚胎作为疾病传播载体的知识，以及我们目前对 SARS-CoV-2 病毒的了解。与 SARS-CoV-2 病毒以及精子、卵子和胚胎相关的未知风险需要重新审视人类体外受精的实施方式。在过去的十年中，冷冻保存和活产结局的改善与透明带破裂程序（例如，囊胚塌陷和活检）有关。反过来，如今在玻璃化和冷冻储存时，胚胎通常不再受到完整透明带的保护。此外，高安全性储存容器已被证明能够抵御潜在的交叉污染，并且可用于常规人类精子冷冻和胚胎玻璃化。提出了几种当前 IVF 实践的选择方案，可以有效降低由于当前 COVID-19 大流行或其他病毒暴露而导致的交叉污染和感染风险。问题仍然存在；在疾病传播风险可能仍然微不足道的情况下，是否需要提高安全性和改变？
