Department of Dermatology, University of Minnesota Medical School, Minneapolis, Minnesota, USA.
Department of Neuroscience, University of Minnesota, Minneapolis, Minnesota, USA.
Exp Dermatol. 2020 Oct;29(10):1004-1011. doi: 10.1111/exd.14165. Epub 2020 Sep 3.
Platelet α-granules release growth factors (GFs) that promote healing and tissue regeneration. Platelet-rich plasma (PRP) is shown to be beneficial in treating alopecia, and however, clinical response can be inconsistent. Due to several fold enrichment of platelets secreting large quantities of GFs following PRP injections, heterogeneity in amounts of GFs secreted by platelets may contribute to inconsistent clinical responses. Herein, we evaluated factors that could potentially contribute to heterogeneous secretion of GFs by platelets. We measured platelet secretion of transforming growth factor beta1 (TGFβ1), platelet-derived growth factor (PDGF-BB), epidermal growth factor (EGF), vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF2) in aliquots of de-identified PRP samples from female patients undergoing therapy in the hair disease clinic. Although secretion of GFs by platelets was comparable in PRP samples of patients with non-cicatricial and cicatricial alopecia, a Shapiro-Wilk test for normal distribution indicated significant variability across all patient samples. The amount of GF secreted by platelets was comparable when PRP prepared from two FDA-cleared devices with distinct techniques were compared. We provide evidence of platelets secreting heterogeneous amounts of GFs within each sample as high and low secretion of random factors could be simultaneously detected. These results suggest inherent heterogeneity in secretion of GFs by platelets in patient samples that are not influenced by the device used to prepare PRP. Since some GFs could have antagonistic effects on hair growth, a balance between amounts of growth promoting and inhibiting factors may be crucial in determining clinical response to PRP therapy.
血小板α颗粒释放生长因子 (GFs)，促进愈合和组织再生。富含血小板的血浆 (PRP) 已被证明对治疗脱发有效，但临床反应可能不一致。由于 PRP 注射后血小板大量分泌 GFs，导致血小板分泌的 GFs 数量存在异质性，这可能是导致临床反应不一致的原因。在此，我们评估了可能导致血小板分泌 GFs 异质性的因素。我们测量了来自女性脱发疾病诊所接受治疗的患者的 PRP 样本等分试样中血小板转化生长因子β 1 (TGFβ1)、血小板衍生生长因子 (PDGF-BB)、表皮生长因子 (EGF)、血管内皮生长因子 (VEGF) 和成纤维细胞生长因子 (FGF2) 的分泌情况。尽管非瘢痕性和瘢痕性脱发患者的 PRP 样本中血小板分泌 GFs 的情况相似，但 Shapiro-Wilk 检验表明所有患者样本的分布均存在显著差异。比较两种具有不同技术的 FDA 批准设备制备的 PRP 样本，血小板分泌的 GF 量相当。我们提供了证据表明，在每个样本中，血小板分泌的 GFs 数量存在异质性，因为可以同时检测到随机因素的高分泌和低分泌。这些结果表明，在不受 PRP 制备设备影响的情况下，患者样本中血小板分泌 GFs 的异质性是固有的。由于某些 GFs 可能对毛发生长有拮抗作用，促进生长和抑制因子的平衡可能是决定 PRP 治疗临床反应的关键。
