在单供体模型中对 5 种富血小板血浆制剂的特性和比较。
Characterization and comparison of 5 platelet-rich plasma preparations in a single-donor model.
机构信息
Cell Culture and Therapy Laboratory, Hôpital de la Conception, AP-HM, Aix-Marseille University, Marseille, France.
Cell Culture and Therapy Laboratory, Hôpital de la Conception, AP-HM, Aix-Marseille University, Marseille, France.
出版信息
Arthroscopy. 2014 May;30(5):629-38. doi: 10.1016/j.arthro.2014.02.020.
PURPOSE
The purpose of this study was to compare the biological characteristics of platelet-rich plasma (PRP) obtained from 4 medical devices and a preparation developed in our laboratory using a single-donor model.
METHODS
Ten healthy persons donated blood that was processed to produce PRP by use of 4 commercial preparation systems and a protocol developed in our laboratory. Volumes and platelet, white blood cell (WBC), and red blood cell concentrations were recorded. The platelet activation status was assessed by flow cytometry. Enzyme-linked immunosorbent assay was used to determine the concentrations of vascular endothelial growth factor, platelet-derived growth factor AB, epidermal growth factor, and transforming growth factor β1. We calculated platelet capture efficiency, relative composition, and increase factors from whole blood in platelets and WBC, as well as platelet and growth factor (GF) doses, provided from each preparation.
RESULTS
Leukocyte-rich PRP was obtained with RegenPRP (RegenLab, Le Mont-sur-Lausanne, Switzerland) and the Mini GPS III System (Biomet Biology, Warsaw, IN) and provides PRP with higher proportions of red blood cells, WBCs, and neutrophils than leukocyte-poor PRP obtained with the Selphyl System (Selphyl, Bethlehem, PA), Arthrex ACP (Arthrex, Naples, FL), and the preparation developed in our laboratory. The highest platelet and GF concentrations and doses were obtained with the Mini GPS III System and the preparation developed in our laboratory. Different centrifugation protocols did not show differences in the percentages of activated platelets. Finally, a positive correlation between platelet doses and all the GFs studied was found, whereas a positive correlation between WBC doses and GFs was found only for vascular endothelial growth factor and epidermal growth factor.
CONCLUSIONS
In a single-donor model, significant biological variations in PRP obtained from different preparation systems were highlighted. The observed differences suggest different results for treated tissue and could explain the large variability in the clinical benefit of PRP reported in the literature.
CLINICAL RELEVANCE
Our findings will help clinicians to choose a system that meets their specific needs for a given indication.
目的
本研究旨在比较从 4 种医疗设备和我们实验室开发的一种制剂获得的富血小板血浆（PRP）的生物学特性，使用单一供体模型。
方法
10 名健康人捐献血液，通过使用 4 种商业制备系统和我们实验室开发的方案对其进行处理以产生 PRP。记录体积和血小板、白细胞（WBC）和红细胞浓度。通过流式细胞术评估血小板激活状态。酶联免疫吸附试验用于测定血管内皮生长因子、血小板衍生生长因子 AB、表皮生长因子和转化生长因子β1 的浓度。我们计算了血小板捕获效率、相对组成和来自全血的血小板和 WBC 的增加因子，以及从每种制剂提供的血小板和生长因子（GF）剂量。
结果
RegenPRP（RegenLab，Le Mont-sur-Lausanne，瑞士）和 Mini GPS III 系统（Biomet Biology，Warsaw，IN）获得富含白细胞的 PRP，与 Selphyl 系统（Selphyl，Bethlehem，PA）、Arthrex ACP（Arthrex，Naples，FL）和我们实验室开发的制剂获得的白细胞减少 PRP 相比，提供更高比例的红细胞、WBC 和中性粒细胞。Mini GPS III 系统和我们实验室开发的制剂获得了最高的血小板和 GF 浓度和剂量。不同的离心方案并未显示出激活血小板百分比的差异。最后，发现血小板剂量与所有研究的 GF 之间呈正相关，而 WBC 剂量与 GF 之间仅呈正相关血管内皮生长因子和表皮生长因子。
结论
在单一供体模型中，从不同制备系统获得的 PRP 存在显著的生物学差异。观察到的差异表明治疗组织的结果不同，这可以解释文献中报道的 PRP 的临床益处存在很大差异。
临床相关性
我们的发现将帮助临床医生根据特定适应症选择满足其特定需求的系统。
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