Zhu Tengfei, Meng Fanzeng, Yao Hao, Wang Yuting, Jiao Xin'an, Yin Yuelan
Yangzhou University, College of Bioscience and Biotechnology/Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses/Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China/Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of Education, Yangzhou 225009, Jiangsu, China.
Sheng Wu Gong Cheng Xue Bao. 2020 Jul 25;36(7):1378-1385. doi: 10.13345/j.cjb.190487.
Listeria monocytogenes (Lm) is zoonotic pathogen that can cause listeriosis, and vaccine is one of the effective methods to prevent this pathogen infection. In this study, we developed a novel vaccine that is a mixture of inactivated bacteria and Montanide™ ISA 61 VG, a mineral oil adjuvant, and evaluated the safety and immune response characteristics of this vaccine. The mice immunized with the ISA 61 VG adjuvant had high safety, and it could induce significantly higher titer of anti-listeriolysin O (LLO) antibody and higher value of IgG2a/IgG1 ratio compared with the group without the adjuvant. In particular, it could provide 100% immune protection against lethal doses of Lm challenge in mice. In summary, ISA 61VG adjuvant significantly enhanced the ability of inactivated listeria vaccine to induce humoral and cellular immune responses, thereby enhanced the protective immune response in the host, and it is a potential vaccine candidate for the prevention of Lm infection in humans and animals.
单核细胞增生李斯特菌（Lm）是一种可导致李斯特菌病的人畜共患病原体，疫苗是预防这种病原体感染的有效方法之一。在本研究中，我们开发了一种新型疫苗，它是灭活细菌与矿物油佐剂Montanide™ ISA 61 VG的混合物，并评估了该疫苗的安全性和免疫反应特征。用ISA 61 VG佐剂免疫的小鼠具有较高的安全性，与未使用佐剂的组相比，它能诱导出显著更高滴度的抗李斯特菌溶血素O（LLO）抗体以及更高的IgG2a/IgG1比值。特别是，它能为小鼠提供针对致死剂量Lm攻击的100%免疫保护。总之，ISA 61VG佐剂显著增强了灭活李斯特菌疫苗诱导体液免疫和细胞免疫反应的能力，从而增强了宿主中的保护性免疫反应，它是预防人和动物Lm感染的一种潜在候选疫苗。
