Food and Beverages Safety Research Center, Urmia University of Medical Sciences, Urmia, Iran.
Microbes Infect. 2010 May;12(5):382-8. doi: 10.1016/j.micinf.2010.02.001. Epub 2010 Feb 10.
We have previously demonstrated the adjuvant activity of naloxone (NLX), a general opioid antagonist, using a DNA vaccine for herpes simplex virus type 1. Here, the adjuvant activity of NLX has been evaluated using a heat-killed Listeria monocytogenes (HKLM) vaccine as a model for general immunization against intracellular bacteria. BALB/c mice were divided into three groups: the Vac group received the HKLM vaccine alone; the NLX-Vac group received the HKLM vaccine in combination with the adjuvant NLX; and the control group received phosphate buffered saline (PBS). Our results indicate that the administration of NLX as an adjuvant enhances the ability of the HKLM vaccine to increase lymphocyte proliferation, delayed type hypersensitivity, and skewing of the immune response toward a T-helper 1 (Th1) pattern. Additionally, combination of NLX with the HKLM vaccine improves protective immunity against L. monocytogenes. In conclusion, administration of NLX as an adjuvant for the HKLM vaccine can enhance cell-mediated immunity and shift the immune response to Th1.
我们之前已经证明了纳洛酮（NLX）作为一种通用阿片类拮抗剂在单纯疱疹病毒 1 DNA 疫苗中的佐剂活性。在这里，我们使用热灭活李斯特菌（HKLM）疫苗作为针对细胞内细菌的一般免疫模型来评估 NLX 的佐剂活性。BALB/c 小鼠分为三组：Vac 组单独接受 HKLM 疫苗；NLX-Vac 组接受与佐剂 NLX 联合的 HKLM 疫苗；对照组接受磷酸盐缓冲盐水（PBS）。我们的结果表明，NLX 作为佐剂的给药增强了 HKLM 疫苗增加淋巴细胞增殖、迟发型超敏反应和免疫应答向 Th1 模式倾斜的能力。此外，NLX 与 HKLM 疫苗联合使用可提高对李斯特菌的保护性免疫。总之，将 NLX 作为 HKLM 疫苗的佐剂给药可以增强细胞介导的免疫并将免疫应答转向 Th1。
