Department of Biomedical Imaging and Radiological Science, China Medical University, Taichung, Taiwan, 404, Republic of China.
School of Chinese Medicine, China Medical University, Taichung, Taiwan, 404, Republic of China.
Macromol Biosci. 2020 Oct;20(10):e2000161. doi: 10.1002/mabi.202000161. Epub 2020 Aug 4.
Uneven distribution of pores, lack of connection between holes, low reproducibility, insufficient mechanical strength, and incomplete volatility of organic solvents are some problems associated with traditional tissue engineering methods for bone defect repair. These characteristics reduce the quality and stability of products. This study uses 3D printing (3DP) to fabricate a biocompatible poly(lactic) acid-based scaffold for repairing bone tissue. Hence, three different types of scaffolds are assessed: a freeze-dried polylactic acid (PLA) scaffold constructed using the traditional freeze-extraction method; a 3D-PLA scaffold produced through the 3DP technique; and a 3D-PLA-bone morphogenetic protein-2 (BMP-2) scaffold that is prepared using 3DP technology, with the addition of BMP-2. To enhance biological activity, polydopamine (pDA) is used to graft BMP-2 on the surface of the 3D-PLA-BMP-2 scaffold. Then, the scaffolds are implanted into the bilateral femoral condyles of rabbits, and their ability to repair the bone tissue defects is tested. The results of the experiments reveal that the 3DP scaffolds are more biocompatible than the ones produced through the traditional manufacturing methods because they enhance cell adhesion and differentiation after pDA modification and BMP-2 fixation. In the future, the 3DP products may be applied for the repair of larger bone defects in the clinical setting.
孔隙分布不均匀、孔之间缺乏连接、重现性低、机械强度不足以及有机溶剂不完全挥发等问题是传统组织工程方法修复骨缺损所面临的一些挑战。这些特性降低了产品的质量和稳定性。本研究使用 3D 打印（3DP）技术制造了一种基于聚乳酸（PLA）的生物相容性支架，用于修复骨组织。因此，评估了三种不同类型的支架：使用传统的冻融提取方法构建的冻干聚乳酸（PLA）支架；通过 3DP 技术生产的 3D-PLA 支架；以及使用 3DP 技术制备的添加了 BMP-2 的 3D-PLA-骨形态发生蛋白-2（BMP-2）支架。为了提高生物活性，将多巴胺（pDA）用于将 BMP-2 接枝到 3D-PLA-BMP-2 支架的表面。然后，将支架植入兔双侧股骨髁中，测试其修复骨组织缺损的能力。实验结果表明，3DP 支架比传统制造方法生产的支架更具生物相容性，因为它们在 pDA 修饰和 BMP-2 固定后增强了细胞黏附和分化。未来，3DP 产品可能会应用于临床修复更大的骨缺损。
