Institute of Biomedical Research and Tissue Engineering, Yangzhou University, Yangzhou, People's Republic of China.
Biomed Mater. 2019 Sep 9;14(6):065003. doi: 10.1088/1748-605X/ab388d.
Repair and regeneration of large bone defects is still a challenge, especially for defects which are the irregular and complex. Three-dimension (3D) printing, as an advanced fabrication technology, has been received considerable attentions due to its high precision, customized geometry and personalization. In this study, 3D porous polylactic acid/nano hydroxyapatite (PLA/nHA) composite scaffolds with enhanced osteogenesis and osteoconductivity were successfully fabricated by desktop fused deposition modeling technology. Morphological, composition and structural analysis revealed that nHA was successfully introduced into the PLA system and homogeneously dispersed in the printed PLA/nHA scaffolds. In vitro antibacterial experiment confirmed that the printed porous PLA/nHA scaffolds have good ability for loading and releasing vancomycin and levofloxacin. Meanwhile, MG-63 cells were used to evaluate the cytocompatibility of printed porous PLA/nHA scaffolds by proliferation and cellular morphological analysis. In addition, rabbit model was established to evaluate the osteogenesis and osteoconductivity of printed PLA/nHA scaffolds. All these results suggested that the 3D printed PLA/nHA scaffolds have great potential for repairing and regeneration of large bone defects.
修复和再生大的骨缺损仍然是一个挑战，特别是对于不规则和复杂的缺损。三维（3D）打印作为一种先进的制造技术，由于其高精度、定制几何形状和个性化而受到了相当多的关注。在这项研究中，通过桌面熔融沉积建模技术成功制备了具有增强成骨和骨传导性的 3D 多孔聚乳酸/纳米羟基磷灰石（PLA/nHA）复合支架。形态学、组成和结构分析表明，nHA 成功地引入到 PLA 体系中，并均匀地分散在打印的 PLA/nHA 支架中。体外抗菌实验证实，打印的多孔 PLA/nHA 支架具有良好的载药和释放万古霉素和左氧氟沙星的能力。同时，通过增殖和细胞形态分析，使用 MG-63 细胞来评估打印的多孔 PLA/nHA 支架的细胞相容性。此外，建立了兔模型来评估打印 PLA/nHA 支架的成骨和成骨传导性。所有这些结果表明，3D 打印的 PLA/nHA 支架在修复和再生大的骨缺损方面具有巨大的潜力。
