Laboratório de Virologia Clínica, Universidade Estadual de Maringá - UEM, Avenida Colombo, 5790, Maringá, Paraná, 87020-900, Brazil.
Laboratório Central do Estado do Paraná - LACEN-PR, Curitiba, Paraná, Brazil.
Braz J Microbiol. 2020 Dec;51(4):1601-1605. doi: 10.1007/s42770-020-00349-3. Epub 2020 Aug 4.
Phylogenetic studies with Zika virus (ZIKV) have been conducted in Brazil. In this study, we sequenced 8 new sequences of the ZIKV envelope (E) gene from strains of cases from the Paraná and Mato Grosso do Sul states in 2016. A low phylogenetic signal was observed, with more than 40% of unresolved quartets, and the Maximum Likelihood Tree grouped all sequences in the Brazilian branches within the Asian genotype. In addition, a Shannon entropy analysis was conducted, showing a high stability in the E protein through the ZIKV polyprotein. Taken together, these results suggest a high degree of conservation in the ZIKV E gene from the recent American outbreaks.
对巴西的寨卡病毒（ZIKV）进行了系统发育研究。在这项研究中，我们对 2016 年来自巴拉那州和南马托格罗索州的病例的 ZIKV 包膜（E）基因的 8 个新序列进行了测序。观察到系统发育信号较低，超过 40%的四联体未解决，最大似然树将所有序列分组在巴西分支内的亚洲基因型中。此外，进行了香农熵分析，表明 ZIKV 多蛋白中 E 蛋白具有高度稳定性。综上所述，这些结果表明，最近美洲暴发的 ZIKV E 基因具有高度保守性。
