Laboratório de Neurodegeneração e Reparo, Departamento de Patologia, Faculdade de Medicina, HUCFF, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Núcleo Multidisciplinar de Pesquisa (Numpex-Bio), Universidade Federal do Rio de Janeiro, Campus Duque de Caxias, Duque de Caxias, RJ 25245-390, Brazil.
Brain Res. 2020 Nov 15;1747:147026. doi: 10.1016/j.brainres.2020.147026. Epub 2020 Aug 1.
Despite the regenerative potential of the Peripheral Nervous System (PNS), injuries with loss of a nerve segment make the functional recovery a challenge. This work aimed to investigate the effects of the association of biodegradable conduits of poly (lactic acid) (PLA) with human adipose-derived stem cells (hADSCs) on the regeneration of the sciatic nerve. C57BL / 6 male mice were submitted to sciatic nerve transection followed by tubulization with PLA conduit. Animals were allocated in two groups: the first received an injection of DMEM inside the conduit (DMEM) and the second received hADSCs inside it (hADSC). Sensory and motor functions were assessed by the pinprick test and electroneuromiography, respectively. To assess neuronal survival the retrograde tracer fluorogold was injected into the sciatic nerve distally to the lesion site. One week after that, animals were sacrificed, tissues harvested and processed for morphological evaluation. After eight weeks, all animals showed sensory recovery in the pinprick test and there was no significant difference between the two groups. The amplitude of the compound muscle action potential was higher in the hADSCs group. The number of myelinated nerve fibers, muscle cells and motor plates was higher in the hADSC group. There was also greater survival of sensory and motor neurons in the hADSC animals. These results suggest that the association of PLA conduit and cell therapy with hADSCs leads to a better functional and morphological recovery after sciatic nerve transection.
尽管周围神经系统 (PNS) 具有再生潜力，但神经节段缺失的损伤使得功能恢复成为一项挑战。本研究旨在探讨聚乳酸 (PLA) 可生物降解导管与人类脂肪来源干细胞 (hADSCs) 联合应用对坐骨神经再生的影响。将 C57BL/6 雄性小鼠进行坐骨神经横断，然后用 PLA 导管进行管化。动物分为两组：第一组在导管内注射 DMEM（DMEM），第二组在导管内注射 hADSCs（hADSC）。通过针刺痛觉试验和电神经肌电图分别评估感觉和运动功能。为了评估神经元存活，将逆行示踪剂荧光金注射到损伤部位远端的坐骨神经中。一周后，处死动物，采集组织并进行形态学评估。八周后，所有动物在针刺痛觉试验中均表现出感觉恢复，两组之间无显著差异。hADSCs 组的复合肌肉动作电位振幅较高。hADSCs 组的有髓神经纤维、肌细胞和运动板数量较高。hADSC 动物的感觉和运动神经元存活也较多。这些结果表明，PLA 导管和细胞治疗与 hADSCs 的联合应用可导致坐骨神经横断后更好的功能和形态恢复。
