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用于快速测定生肉和加工肉制品细菌学质量的自动比浊法。

Automated turbidimetry for rapid determination of the bacteriological quality of raw meat and processed meat products.

作者信息

Schulz E, Jensen B, Celerynova E

机构信息

Danish Consumers Cooperative Society, Albertslund.

出版信息

Int J Food Microbiol. 1988 May;6(3):219-27. doi: 10.1016/0168-1605(88)90014-1.

DOI:10.1016/0168-1605(88)90014-1
PMID:3275300
Abstract

A fully automated centrifugal spectrophotometer for turbidimetric measurements linked to a computer for data processing was used to record and analyze growth of different types of bacteria associated with spoilage of raw meat and processed meat products (sliced meat for sandwiches) in order to predict bacterial counts. Media for this specific analysis were developed to enumerate total viable count, lactic acid bacteria. Brochothrix thermosphacta and Pseudomonas spp. Correlation coefficients of -0.91 to -0.97 were obtained between the conventional plate count technique and the turbidimetric method (linear regression). Lactic acid bacteria in the range 10(3) to 10(9) cfu/g could be detected within 48 h, whereas total viable count, Brochothrix thermosphacta and Pseudomonas spp. generally could be detected within 24 h. Counts exceeding 10(7) cfu/g could be detected within 6 h.

摘要

一台用于比浊法测量的全自动离心分光光度计与一台用于数据处理的计算机相连,用于记录和分析与生肉和加工肉制品(三明治切片肉)腐败相关的不同类型细菌的生长情况,以便预测细菌数量。为进行这种特定分析而开发的培养基用于计数总活菌数、乳酸菌、热杀索丝菌和假单胞菌属。传统平板计数技术与比浊法(线性回归)之间的相关系数为-0.91至-0.97。在48小时内可检测到10³至10⁹ cfu/g范围内的乳酸菌,而总活菌数、热杀索丝菌和假单胞菌属通常在24小时内可被检测到。超过10⁷ cfu/g的菌数在6小时内可被检测到。

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