Montani M, Vaamonde G, Resnik S L, Buera P
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Int J Food Microbiol. 1988 Oct;7(2):115-22. doi: 10.1016/0168-1605(88)90004-9.
To study the temperature influence on both Penicillium citrinum growth and citrinin accumulation, a 20, 25 and 30 degrees C. Radial and 30 degrees C. Radial growth rate and lag phase were determined from the increase in colony diameter with time. The optimal temperature for P. citrinum growth was 30 degrees C. Citrinin extracted from the agar medium was determined by thin layer chromatography. Citrinin accumulation kinetics were analyzed by fitting the data to curves generated by using a logistic function. The parameters obtained from this equation demonstrated, for all temperatures studied, that the maximum citrinin accumulation by P. citrinum on Czapek agar with maize extract was at about 30 degrees C. At 37 degrees C a rapid decrease in the citrinin concentration was observed after a maximal value was reached.
为研究温度对桔青霉生长和桔霉素积累的影响，设置了20℃、25℃和30℃三个温度条件。通过菌落直径随时间的增加来测定径向生长速率和30℃时的径向生长速率以及延迟期。桔青霉生长的最适温度为30℃。从琼脂培养基中提取的桔霉素通过薄层色谱法进行测定。通过将数据拟合到使用逻辑函数生成的曲线来分析桔霉素积累动力学。从该方程获得的参数表明，在所研究的所有温度下，桔青霉在含有玉米提取物的察氏琼脂上最大桔霉素积累量约在30℃。在37℃时，达到最大值后观察到桔霉素浓度迅速下降。
