Liu Yanjie, Duan Anqing, Chen Longfei, Wang Dan, Xie Qiaohong, Xiang Biyun, Lin Yamin, Hao Xiaoran, Zhu Xudong
Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University, Beijing, China.
Zhejiang Medicine Co., Ltd., Zhejiang, China.
Front Microbiol. 2020 Jul 10;11:1426. doi: 10.3389/fmicb.2020.01426. eCollection 2020.
A conserved open reading frame, , is described in , sharing a remarkable similarity with fungal diterpene synthases whose function is less studied. Loss-of-function approach manifested that was necessary for the growth and the development of the fungus. A deletion strain, Δ, showed a fundamental retardation in growth, which could deliberately be restored by the addition of exogenous sterols to the media. Gas chromatography-mass spectrometry analysis confirmed the loss of the ability to produce certain sterols. Thus, the tolerance and the resistance of Δ to several stress conditions were impaired. Secondary metabolites, such as the polyketide derivative dibenzodioxocinones, were significantly diminished. At the molecular level, the deletion of even affected the expression of genes in the mevalonate pathway. This report adds knowledge about fungal diterpene synthases in .
在[具体文献]中描述了一个保守的开放阅读框[具体名称]，它与功能研究较少的真菌二萜合酶具有显著相似性。功能丧失方法表明[具体名称]对于真菌的生长和发育是必需的。缺失菌株Δ[具体名称]表现出生长的基本迟缓，通过向培养基中添加外源甾醇可以有意地恢复这种迟缓。气相色谱 - 质谱分析证实了产生某些甾醇能力的丧失。因此，Δ[具体名称]对几种胁迫条件的耐受性和抗性受损。次级代谢产物，如聚酮衍生物二苯并二恶英酮，显著减少。在分子水平上，[具体名称]的缺失甚至影响了甲羟戊酸途径中基因的表达。本报告增加了关于[具体文献]中真菌二萜合酶的知识。
