Wang Dan, Akhberdi Oren, Hao Xiaoran, Yu Xi, Chen Longfei, Liu Yanjie, Zhu Xudong
National Key Program of Microbiology and Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, China.
National Experimental Teaching Demonstrating Center, School of Life Sciences, Beijing Normal University, Beijing, China.
Front Microbiol. 2017 Sep 27;8:1879. doi: 10.3389/fmicb.2017.01879. eCollection 2017.
The canonical Gcn2/Cpc1 kinase in fungi coordinates the expression of target genes in response to amino acid starvation. To investigate its possible role in secondary metabolism, we characterized a homolog in the taxol-producing fungus . Deletion of the gene led to severe physiological defects under amino acid starvation, suggesting a conserved function of in amino acid sensing. The mutant strain displayed retardation in vegetative growth. It generated dramatically fewer conidia, suggesting a connection between amino acid metabolism and conidiation in this fungus. Importantly, disruption of the gene altered the production of secondary metabolites by HPLC profiling. For instance, under amino acid starvation, the deletion strain barely produced secondary metabolites including the known natural product pestalotiollide B. Even more, we showed that played critical roles in the tolerance to several stress conditions. exhibited a hypersensitivity to Calcofluor white and Congo red, implying a role of Gcn2 in maintaining the integrity of the cell wall. This study suggests that Gcn2 kinase is an important global regulator in the growth and development of filamentous fungi and will provide knowledge for the manipulation of secondary metabolism in . .
真菌中的典型Gcn2/Cpc1激酶可响应氨基酸饥饿来协调靶基因的表达。为了研究其在次级代谢中的可能作用，我们对产紫杉醇真菌中的一个同源物进行了表征。该基因的缺失导致在氨基酸饥饿条件下出现严重的生理缺陷，表明其在氨基酸感应方面具有保守功能。突变菌株在营养生长方面表现出迟缓。它产生的分生孢子显著减少，这表明该真菌中氨基酸代谢与分生孢子形成之间存在联系。重要的是，通过高效液相色谱分析，该基因的破坏改变了次级代谢产物的产生。例如，在氨基酸饥饿条件下，缺失菌株几乎不产生包括已知天然产物pestalotiollide B在内的次级代谢产物。此外，我们表明它在对几种胁迫条件的耐受性中起关键作用。它对荧光增白剂和刚果红表现出超敏反应，这意味着Gcn2在维持细胞壁完整性方面发挥作用。这项研究表明，Gcn2激酶是丝状真菌生长和发育中的重要全局调节因子，并将为操纵[具体真菌名称]中的次级代谢提供知识。
