Selvanayagam P, Blick M, Narni F, van Tuinen P, Ledbetter D H, Alexanian R, Saunders G F, Barlogie B
Department of Hematology, University of Texas System Cancer Center, M. D. Anderson Hospital and Tumor Institute, Houston 77030.
Blood. 1988 Jan;71(1):30-5.
Structural alterations of the c-myc oncogene in human Burkitt's lymphoma and mouse plasmacytoma suggest that this oncogene is involved in several B cell neoplasms. The possibility of c-myc alterations in human myeloma has not been explored, probably because the low proliferative activity characteristic of this tumor impairs the propagation of representative cell lines for the performance of adequate cytogenetic studies. This report describes alterations in the c-myc locus with concomitant elevated expression of mRNA in the tumor cells of two of 37 patients with multiple myeloma. In one case, somatic cell hybrid studies revealed that the cloned rearranged DNA was entirely derived from chromosome 8, thus indicating a novel mechanism of c-myc activation different from that in Burkitt's lymphoma. Seven other patients exhibited five- to 12-fold overexpression of c-myc RNA when compared with normal marrow cells. Elevated mRNA expression in about one fourth of our patients suggests that the c-myc oncogene has a pathogenetic role in the evolution of multiple myeloma.
人类伯基特淋巴瘤和小鼠浆细胞瘤中c-myc癌基因的结构改变表明，该癌基因与多种B细胞肿瘤有关。尚未探讨人类骨髓瘤中c-myc改变的可能性，这可能是因为该肿瘤的低增殖活性特征妨碍了用于进行充分细胞遗传学研究的代表性细胞系的增殖。本报告描述了37例多发性骨髓瘤患者中有2例的肿瘤细胞中c-myc基因座的改变以及mRNA表达的同时升高。在1例病例中，体细胞杂交研究显示克隆的重排DNA完全来自8号染色体，从而表明c-myc激活的一种新机制不同于伯基特淋巴瘤中的机制。与正常骨髓细胞相比，其他7例患者的c-myc RNA表达高出5至12倍。约四分之一的患者中mRNA表达升高表明c-myc癌基因在多发性骨髓瘤的发生发展中具有致病作用。
