Nie Hongtao, Jiang Liwen, Huo Zhongming, Liu Lianhui, Yang Feng, Yan Xiwu
Engineering and Technology Research Center of Shellfish Breeding in Liaoning Province, Dalian Ocean University, Dalian, 116023, China; Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian, 116023, China.
Engineering and Technology Research Center of Shellfish Breeding in Liaoning Province, Dalian Ocean University, Dalian, 116023, China.
Fish Shellfish Immunol. 2016 Aug;55:358-66. doi: 10.1016/j.fsi.2016.06.008. Epub 2016 Jun 7.
The Manila clam, Ruditapes philippinarum, is an economically important shellfish in marine aquaculture, with a broad thermal tolerance. The ability to cope with cold stress is quite important for the survival of aquatic species under natural conditions. A cold-tolerant clam that can survive the winter at temperatures below 0 °C might extend our understanding of the mechanisms underlying the response to cold stress. In this study, the transcriptional response of the Manila clam to cold stress (-1 °C) was characterized using RNA sequencing. The transcriptomes of a cold-treatment (O) group of clams, which survived under cold stress, and the control group (OC2), which was not subjected to cold stress, were sequenced with the Illumina HiSeq platform. In all, 148,593 unigenes were generated. Compared with the unigene expression profile of the control group, 1760 unigenes were up regulated and 2147 unigenes were down regulated in the O group. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses revealed that signal transduction, mitochondrial metabolism, cellular component organization or biogenesis, and energy production processes were the most highly enriched pathways among the genes that were differentially expressed under cold stress. All these pathways could be assigned to the following biological functions in the cold-tolerant Manila clam: signal response to cold stress, antioxidant response, cell proliferation, and energy production.
菲律宾蛤仔（Ruditapes philippinarum）是海水养殖中具有重要经济价值的贝类，具有广泛的热耐受性。应对冷应激的能力对于水生物种在自然条件下的生存非常重要。一种能够在0°C以下的温度下越冬的耐寒蛤仔可能会拓展我们对冷应激反应潜在机制的理解。在本研究中，利用RNA测序对菲律宾蛤仔对冷应激（-1°C）的转录反应进行了表征。使用Illumina HiSeq平台对在冷应激下存活的冷处理（O）组蛤仔和未经历冷应激的对照组（OC2）的转录组进行了测序。总共产生了148,593个单基因。与对照组的单基因表达谱相比，O组中有1760个单基因上调，2147个单基因下调。基因本体论和京都基因与基因组百科全书分析表明，信号转导、线粒体代谢、细胞组分组织或生物发生以及能量产生过程是冷应激下差异表达基因中富集程度最高的途径。所有这些途径在耐寒菲律宾蛤仔中可归因于以下生物学功能：对冷应激的信号反应、抗氧化反应、细胞增殖和能量产生。
