Hernandez M R, Igoe F, Neufeld A H
Ophthalmic Pharmacology Unit, Eye Research Institute, Boston, MA 02114.
Invest Ophthalmol Vis Sci. 1988 Jan;29(1):78-89.
The extracellular matrix of the lamina cribrosa may be important in the changes in the optic nerve head associated with glaucoma. To investigate the cell biology of this tissue, human lamina cribrosa was explanted in tissue culture and two cell types grown from this tissue were characterized. The most common cell type obtained was a large, flat, polygonal cell which was negative for glial fibrillar acidic protein (GFAP) and could be serially subcultured. This cell type synthesized collagens type III and type IV, fibronectin and elastin. Much less commonly grown was a cell type with conspicuous long processes and which was positive for GFAP. This presumed astrocyte synthesized collagen type IV and fibronectin. Fibroblastic cells were not obtained from this tissue but were easily grown from sclera. The cells that we have cultured from the human lamina cribrosa may produce the extracellular matrix present in the cribriform plates of this tissue and be important in the glaucomatous process.
筛板的细胞外基质可能在与青光眼相关的视神经乳头变化中起重要作用。为了研究该组织的细胞生物学，将人筛板植入组织培养中，并对从该组织生长出的两种细胞类型进行了表征。获得的最常见细胞类型是大的、扁平的多边形细胞，其胶质纤维酸性蛋白（GFAP）呈阴性，并且可以连续传代培养。这种细胞类型合成III型和IV型胶原、纤连蛋白和弹性蛋白。生长较少见的是一种具有明显长突起且GFAP呈阳性的细胞类型。这种推测的星形胶质细胞合成IV型胶原和纤连蛋白。未从该组织获得成纤维细胞，但很容易从巩膜中生长出来。我们从人筛板培养的细胞可能产生该组织筛状板中存在的细胞外基质，并在青光眼过程中起重要作用。
