BIOFORGE (Group for Advanced Materials and Nanobiotechnology), CIBER-BBN University of Valladolid, 47011 Valladolid, Spain.
BIOFORGE (Group for Advanced Materials and Nanobiotechnology), CIBER-BBN University of Valladolid, 47011 Valladolid, Spain.
Int J Biol Macromol. 2020 Dec 1;164:1640-1648. doi: 10.1016/j.ijbiomac.2020.08.004. Epub 2020 Aug 3.
One of the main goals in both tissue engineering and regenerative medicine is to design innovative synthetic scaffolds that can simulate and control the communication pathways between cells and the extracellular matrix (ECM). In this context, we describe herein the characterization of protein polymer, a recombinant elastin-like recombinamer (ELR) designed for developing tissue-engineered devices for use in vascular regeneration. This ELR is composed of an elastin-like backbone that contains a fibronectin domain, which provides specific, endothelial cell adhesion, and a protease target domain directed towards specific proteases involved in ECM remodeling. We also compare the specific response of endothelial and fibroblast cells to ELR scaffolds and show that cell adhesion and spreading on this ELR is significantly higher for endothelial cells than for fibroblasts. The reactivity of this polymer and its hydrogels to specific enzymatic degradation is demonstrated in vitro. As with natural elastin, enzymatic hydrolysis of the ELR produces elastin-derived peptides, or "matrikines", which, in turn, are potentially able to regulate important cell activities.
在组织工程和再生医学中，主要目标之一是设计创新的合成支架，以模拟和控制细胞与细胞外基质（ECM）之间的通讯途径。在这方面，我们描述了蛋白质聚合物的特性，这是一种为开发用于血管再生的组织工程设备而设计的重组弹性蛋白样重组体（ELR）。这种 ELR 由弹性蛋白样骨架组成，其中包含一个纤维连接蛋白结构域，该结构域提供内皮细胞特异性粘附，并提供一个针对特定参与 ECM 重塑的蛋白酶的靶结构域。我们还比较了内皮细胞和成纤维细胞对 ELR 支架的特定反应，并表明内皮细胞在这种 ELR 上的粘附和扩展明显高于成纤维细胞。该聚合物及其水凝胶对特定酶降解的反应性在体外得到了证明。与天然弹性蛋白一样，ELR 的酶水解产生弹性蛋白衍生肽，或“基质细胞因子”，它们反过来可能能够调节重要的细胞活动。
