• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

酿酒酵母SUP53 tRNA基因转录本在体外可被哺乳动物细胞提取物加工，但在体内不能被加工。

Saccharomyces cerevisiae SUP53 tRNA gene transcripts are processed by mammalian cell extracts in vitro but are not processed in vivo.

作者信息

Ganguly S, Sharp P A, RajBhandary U L

机构信息

Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.

出版信息

Mol Cell Biol. 1988 Jan;8(1):361-70. doi: 10.1128/mcb.8.1.361-370.1988.

DOI:10.1128/mcb.8.1.361-370.1988
PMID:3275875
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC363131/
Abstract

We describe the results of our studies of expression of a Saccharomyces cerevisiae amber suppressor tRNA(Leu) gene (SUP53) in mammalian cells in vivo and in cell extracts in vitro. Parallel studies were carried out with the wild-type (Su-) tRNA(Leu) gene. Extracts from HeLa or CV1 cells transcribed both tRNA(Leu) genes. The transcripts were processed correctly at the 5' and 3' ends and accurately spliced to produce mature tRNA(Leu). Surprisingly, when the same tRNA(Leu) genes were introduced into CV1 cells, only pre-tRNAs(Leu) were produced. The pre-tRNAs(Leu) made in vivo were of the same size and contained the 5'-leader and 3'-trailer sequences as did pre-tRNAs(Leu) made in vitro. Furthermore, the pre-tRNAs(Leu) made in vivo were processed to mature tRNA(Leu) when incubated with HeLa cell extracts. A tRNA(Leu) gene from which the intervening sequence had been removed yielded RNAs that also were not processed at either their 5' or 3' termini. Thus, processing of pre-tRNA(Leu) in CV1 cells is blocked at the level of 5'- and 3'-end maturation. One possible explanation of the discrepancy in the results obtained in vivo and in vitro is that tRNA biosynthesis in mammalian cells involves transport of pre-tRNA from the site of its synthesis to a site or sites where processing takes place, and perhaps the yeast pre-tRNAs(Leu) synthesized in CV1 cells are not transported to the appropriate site.

摘要

我们描述了酿酒酵母琥珀抑制tRNA（Leu）基因（SUP53）在哺乳动物细胞体内和体外细胞提取物中的表达研究结果。同时对野生型（Su-）tRNA（Leu）基因进行了平行研究。来自HeLa或CV1细胞的提取物转录了这两种tRNA（Leu）基因。转录本在5'和3'末端进行了正确加工，并准确剪接以产生成熟的tRNA（Leu）。令人惊讶的是，当将相同的tRNA（Leu）基因导入CV1细胞时，只产生了前体tRNA（Leu）。体内产生的前体tRNA（Leu）大小相同，并且含有与体外产生的前体tRNA（Leu）相同的5'前导序列和3'尾随序列。此外，当与HeLa细胞提取物一起孵育时，体内产生的前体tRNA（Leu）被加工成成熟的tRNA（Leu）。一个去除了间隔序列的tRNA（Leu）基因产生的RNA在其5'或3'末端也没有被加工。因此，CV1细胞中前体tRNA（Leu）的加工在5'和3'末端成熟水平上被阻断。体内和体外结果差异的一种可能解释是，哺乳动物细胞中的tRNA生物合成涉及前体tRNA从其合成位点转运到一个或多个加工位点，也许在CV1细胞中合成的酵母前体tRNA（Leu）没有被转运到合适的位点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ff/363131/c9ef2357636f/molcellb00061-0390-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ff/363131/ada2e159ba66/molcellb00061-0386-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ff/363131/ddcd6d528fbe/molcellb00061-0387-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ff/363131/46a52c95e1b6/molcellb00061-0387-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ff/363131/8fccd643440e/molcellb00061-0388-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ff/363131/8ba4496e3571/molcellb00061-0388-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ff/363131/fa69aaa02758/molcellb00061-0389-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ff/363131/320ece068036/molcellb00061-0389-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ff/363131/ae626d9df3ad/molcellb00061-0390-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ff/363131/8c740e69310b/molcellb00061-0390-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ff/363131/c9ef2357636f/molcellb00061-0390-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ff/363131/ada2e159ba66/molcellb00061-0386-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ff/363131/ddcd6d528fbe/molcellb00061-0387-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ff/363131/46a52c95e1b6/molcellb00061-0387-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ff/363131/8fccd643440e/molcellb00061-0388-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ff/363131/8ba4496e3571/molcellb00061-0388-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ff/363131/fa69aaa02758/molcellb00061-0389-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ff/363131/320ece068036/molcellb00061-0389-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ff/363131/ae626d9df3ad/molcellb00061-0390-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ff/363131/8c740e69310b/molcellb00061-0390-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ff/363131/c9ef2357636f/molcellb00061-0390-c.jpg

相似文献

1
Saccharomyces cerevisiae SUP53 tRNA gene transcripts are processed by mammalian cell extracts in vitro but are not processed in vivo.酿酒酵母SUP53 tRNA基因转录本在体外可被哺乳动物细胞提取物加工，但在体内不能被加工。
Mol Cell Biol. 1988 Jan;8(1):361-70. doi: 10.1128/mcb.8.1.361-370.1988.
2
Effect of intron mutations on processing and function of Saccharomyces cerevisiae SUP53 tRNA in vitro and in vivo.内含子突变对酿酒酵母SUP53 tRNA体外及体内加工与功能的影响。
Mol Cell Biol. 1986 Jul;6(7):2663-73. doi: 10.1128/mcb.6.7.2663-2673.1986.
3
Intron mutations affect splicing of Saccharomyces cerevisiae SUP53 precursor tRNA.内含子突变影响酿酒酵母SUP53前体tRNA的剪接。
Mol Cell Biol. 1986 Jul;6(7):2674-83. doi: 10.1128/mcb.6.7.2674-2683.1986.
4
Effects of tRNA-intron structure on cleavage of precursor tRNAs by RNase P from Saccharomyces cerevisiae.酵母核糖核酸酶P切割前体tRNA时tRNA内含子结构的影响
Nucleic Acids Res. 1988 Mar 25;16(6):2537-52. doi: 10.1093/nar/16.6.2537.
5
Mutations in the anticodon stem affect removal of introns from pre-tRNA in Saccharomyces cerevisiae.反密码子茎中的突变影响酿酒酵母前体tRNA中内含子的去除。
Mol Cell Biol. 1989 Oct;9(10):4220-8. doi: 10.1128/mcb.9.10.4220-4228.1989.
6
Transfer RNA post-transcriptional processing, turnover, and subcellular dynamics in the yeast Saccharomyces cerevisiae.酵母酿酒酵母中转录 RNA 的转录后加工、周转和亚细胞动态。
Genetics. 2013 May;194(1):43-67. doi: 10.1534/genetics.112.147470.
7
Intron sequence and structure requirements for tRNA splicing in Saccharomyces cerevisiae.酿酒酵母中tRNA剪接的内含子序列和结构要求。
J Biol Chem. 1988 Sep 25;263(27):13839-47.
8
Endonucleolytic cleavage of a long 3'-trailer sequence in a nuclear yeast suppressor tRNA.核酵母抑制性tRNA中长3'尾序列的核酸内切酶切割
Biochemistry. 1992 Nov 10;31(44):10817-24. doi: 10.1021/bi00159a024.
9
A yeast tRNA(Arg) gene can act as promoter for a 5' flank deficient, non-transcribable tRNA(SUP)6 gene to produce biologically active suppressor tRNA.一个酵母tRNA（精氨酸）基因可作为5'侧翼缺失、不可转录的tRNA（SUP）6基因的启动子，以产生具有生物活性的抑制性tRNA。
Nucleic Acids Res. 1988 Apr 11;16(7):2841-57. doi: 10.1093/nar/16.7.2841.
10
Transcription and processing of a yeast tRNA gene containing a modified intervening sequence.一个含有修饰性间隔序列的酵母tRNA基因的转录与加工
Proc Natl Acad Sci U S A. 1980 May;77(5):2564-8. doi: 10.1073/pnas.77.5.2564.

引用本文的文献

1
Wheat germ splicing endonuclease is highly specific for plant pre-tRNAs.小麦胚芽剪接内切核酸酶对植物前体tRNA具有高度特异性。
EMBO J. 1988 Dec 1;7(12):3823-8. doi: 10.1002/j.1460-2075.1988.tb03267.x.
2
In vivo pre-tRNA processing in Saccharomyces cerevisiae.酿酒酵母中的体内前体tRNA加工
Mol Cell Biol. 1991 Jan;11(1):425-39. doi: 10.1128/mcb.11.1.425-439.1991.

本文引用的文献

1
Bacterio-opsin mRNA in wild-type and bacterio-opsin-deficient Halobacterium halobium strains.野生型和菌视紫红质缺陷型盐沼盐杆菌中的菌视紫红质 mRNA。
Proc Natl Acad Sci U S A. 1984 Jan;81(1):125-9. doi: 10.1073/pnas.81.1.125.
2
Nucleotide sequence of "renaturable" leucine transfer ribonucleic acid.
FEBS Lett. 1971 Oct 1;17(2):265-268. doi: 10.1016/0014-5793(71)80161-8.
3
Isolation and sequence determination of the 3'-terminal regions of isotopically labelled RNA molecules.同位素标记RNA分子3'末端区域的分离与序列测定
Nucleic Acids Res. 1974 May;1(5):653-71. doi: 10.1093/nar/1.5.653.
4
Construction of a functional human suppressor tRNA gene: an approach to gene therapy for beta-thalassaemia.功能性人类抑制性tRNA基因的构建：一种β地中海贫血基因治疗方法
Nature. 1982 Apr 8;296(5857):537-40. doi: 10.1038/296537a0.
5
Yeast tRNA3Leu gene transcribed and spliced in a HeLa cell extract.酵母tRNA3亮氨酸基因在HeLa细胞提取物中进行转录和剪接。
Proc Natl Acad Sci U S A. 1981 Oct;78(10):5963-7. doi: 10.1073/pnas.78.10.5963.
6
Establishment of mammalian cell lines containing multiple nonsense mutations and functional suppressor tRNA genes.建立含有多个无义突变和功能性抑制性tRNA基因的哺乳动物细胞系。
Cell. 1982 Nov;31(1):137-46. doi: 10.1016/0092-8674(82)90413-5.
7
Characterization of tRNA precursor splicing in mammalian extracts.哺乳动物提取物中tRNA前体剪接的特征分析。
J Biol Chem. 1983 Oct 10;258(19):11974-80.
8
Yeast amber suppressors corresponding to tRNA3Leu genes.对应于tRNA3Leu基因的酵母琥珀抑制基因。
J Mol Biol. 1984 Sep 15;178(2):209-26. doi: 10.1016/0022-2836(84)90140-2.
9
An amber suppressor tRNA gene derived by site-specific mutagenesis: cloning and function in mammalian cells.通过位点特异性诱变获得的琥珀抑制tRNA基因：在哺乳动物细胞中的克隆与功能
Proc Natl Acad Sci U S A. 1982 Oct;79(19):5813-7. doi: 10.1073/pnas.79.19.5813.
10
Nucleotide sequence of yeast LEU2 shows 5'-noncoding region has sequences cognate to leucine.酵母LEU2的核苷酸序列表明，5'-非编码区具有与亮氨酸同源的序列。
Cell. 1982 Dec;31(2 Pt 1):319-25. doi: 10.1016/0092-8674(82)90125-8.