Souza Jose Moises, Tuin Stephen A, Robinson Adam G, Souza Joao Gustavo Oliveira de, Bianchini Marco Aurelio, Miguez Patricia A
Centro de Ciências da Saúde, Departamento de Odontologia, Campus Reitor David Ferreira Lima, Universidade Federal de Santa Catarina, Bairro Trindade, Florianópolis 88040-970, Brazil.
Oral and Craniofacial Health Sciences, Adams School of Dentistry, Koury Oral Health Sciences Building, Rm 4608, CB# 7455, University of North Carolina at Chapel Hill, 385 South Columbia Street, Chapel Hill, NC 27599-7455, USA.
Dent J (Basel). 2020 Aug 4;8(3):86. doi: 10.3390/dj8030086.
We investigated the effects of two common dietary supplements on bone healing in dental extraction sockets in humans. In this randomized pilot trial, male subjects took Grape Seed Extract [GSE] or Grapefruit Extract [GFE] starting two weeks prior to dental extraction and maintained this regimen for sixty days after surgery. Extraction sockets were filled with a collagen plug. After 24 h, a socket sample was collected and processed for quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) and an 84-gene wound healing assay. Sixty days after tooth extraction, a core of newly formed bone was obtained prior to dental implant placement and processed for histology. qRT-PCR revealed that GFE led to a significant decrease in platelet-derived growth factor and interleukin (IL)1-β compared to GSE, and a significant decrease in IL-6 and CXCL2 compared to control. GSE led to a significant increase in coagulation factor Von Willebrand and inflammatory marker IL1-β compared to GFE. WISP1 and CXCL5 were upregulated in both groups. Overall, GFE showed a downregulation of inflammation and GSE led to a decrease in collagen density and increased osteoclasts. This pilot trial highlights the need for further investigation on the mechanism of action of such supplements on bone healing and oral health.
我们研究了两种常见膳食补充剂对人类拔牙窝骨愈合的影响。在这项随机试点试验中，男性受试者在拔牙前两周开始服用葡萄籽提取物[GSE]或葡萄柚提取物[GFE]，并在术后维持该方案60天。拔牙窝用胶原塞填充。24小时后，收集拔牙窝样本并进行定量实时逆转录聚合酶链反应(qRT-PCR)和84基因伤口愈合分析。拔牙60天后，在植入牙种植体之前获取新形成骨的核心并进行组织学处理。qRT-PCR显示，与GSE相比，GFE导致血小板衍生生长因子和白细胞介素(IL)1-β显著降低，与对照组相比，IL-6和CXCL2显著降低。与GFE相比，GSE导致凝血因子血管性血友病因子和炎症标志物IL1-β显著增加。两组中WISP1和CXCL5均上调。总体而言，GFE显示炎症下调，GSE导致胶原密度降低和破骨细胞增加。这项试点试验凸显了进一步研究此类补充剂对骨愈合和口腔健康作用机制的必要性。
