Skotniczny Magdalena, Satora Paweł, Pańczyszyn Katarzyna, Cioch-Skoneczny Monika
Department of Fermentation Technology and Microbiology, University of Agriculture in Krakow, Balicka 122, 30-149 Krakow, Poland.
Foods. 2020 Aug 4;9(8):1054. doi: 10.3390/foods9081054.
The influence of fruit varieties on yeast ecology during spontaneous plum mash fermentation was investigated. Yeast colonies were isolated from mashes obtained from four plum varieties throughout fermentation in laboratory conditions during two consecutive years. The yeast strains were differentiated by random amplification of polymorphic DNA (RAPD-PCR) and identified by the 26S rDNA D1/D2 sequence analysis. , spp. and were the dominant yeasts during the early stages of plum mash fermentation, while the middle and end phases were dominated by . The strains of , , , and were also detected in fermenting plum mashes. sp. M1, H1 and H2 strains were detected in all samples, irrespective of the tested variety and year. Investigation of the impact of individual yeast strains on the production of volatile compounds showed the potential possibility of using them as starter cultures.
研究了水果品种对李子自然发酵醪液发酵过程中酵母生态的影响。连续两年在实验室条件下,从四种李子品种的发酵醪液中分离出酵母菌落。通过随机扩增多态性DNA(RAPD-PCR)对酵母菌株进行区分,并通过26S rDNA D1/D2序列分析进行鉴定。在李子发酵醪液发酵初期, 、 属和 属是优势酵母,而在中期和后期则以 属为主。在发酵的李子醪液中也检测到了 、 、 、 属和 属的菌株。无论测试的品种和年份如何,在所有样品中均检测到 属M1菌株、 属H1和H2菌株。对单个酵母菌株对挥发性化合物产生影响的研究表明,有可能将它们用作发酵剂。