Golińska Edyta, Strus Magdalena, Tomusiak-Plebanek Anna, Więcek Grażyna, Kozień Łucja, Lauterbach Ryszard, Pawlik Dorota, Rzepecka-Węglarz Beata, Kędzierska Jolanta, Dorycka Małgorzata, Heczko Piotr B
Jagiellonian University Medical College, 31-121 Cracow, Poland.
Department of Neonatology, Jagiellonian University, Medical College, 31-501 Cracow, Poland.
J Clin Med. 2020 Aug 4;9(8):2517. doi: 10.3390/jcm9082517.
There are only a few reports in the literature about translocation of coagulase-negative staphylococci (CoNS) as a primary cause of sepsis in neonates, although CoNS are among a short list of "translocating" bacteria when present in abundance.
468 blood samples, 119 stool samples, and 8 catheter tips, from 311 neonates, were tested for presence of microorganisms. CoNS strains isolated from the blood and stool or from blood and catheter tip of the same newborn at approximately the same time were paired and typed with PFGE (Pulse-Field Gel Electrophoresis) method. The strains were then tested for the presence of adherence genes and biofilm formation.
The strains with identical PFGE profiles in comparison to those with non-identical profiles differed in terms of the pattern of the virulence genes and showed a lack of the genes related to adherence, but more often presence of IS256, which is related to virulence. They also were phenotypically unable to adhere to intestinal Caco2 cells.
A considerable proportion of CoNS strains isolated from bloodstream of VLBW/LWB neonates was identical to the strains isolated from faeces of the same neonates at the same time. These observations may offer indirect evidence indicating that at least some CoNS can translocate from the gastrointestinal tract of the premature neonates into the bloodstream and thus cause generalized infection.
尽管凝固酶阴性葡萄球菌（CoNS）大量存在时属于“易位”细菌的少数几种，但关于其作为新生儿败血症主要病因易位的文献报道很少。
对311名新生儿的468份血样、119份粪便样本和8个导管尖端进行微生物检测。从同一新生儿几乎同一时间采集的血液和粪便或血液和导管尖端分离出的CoNS菌株进行配对，并用脉冲场凝胶电泳（PFGE）方法分型。然后检测这些菌株是否存在黏附基因和生物膜形成。
与PFGE图谱不同的菌株相比，具有相同PFGE图谱的菌株在毒力基因模式方面存在差异，显示缺乏与黏附相关的基因，但更常存在与毒力相关的IS256。它们在表型上也无法黏附于肠道Caco2细胞。
从极低出生体重/低出生体重新生儿血液中分离出的相当一部分CoNS菌株与同一新生儿同时从粪便中分离出的菌株相同。这些观察结果可能提供间接证据，表明至少一些CoNS可以从早产新生儿的胃肠道易位到血液中，从而引起全身感染。
