Chan J K, Kung I T
Institute of Pathology, Queen Elizabeth Hospital, Hong Kong.
Am J Clin Pathol. 1988 Jan;89(1):30-4. doi: 10.1093/ajcp/89.1.30.
In fine-needle aspiration cytologic examination, nuclear features are often better assessed in hematoxylin and eosin (H and E) or Papanicolaou- (Pap) stained than Romanowsky-stained smears. However, both H and E and Pap stains require the use of immediately wet-fixed smears for cytomorphologic preservation. Some degree of air drying is usually inevitable. Placing air-dried smears in normal saline for 30 seconds before fixation in 95% alcohol is found to be a simple means of rehydrating the cells. The quality of the rehydrated smears is superior or identical to that of the immediately wet-fixed smears, provided that the period of drying does not exceed 30 minutes.
在细针穿刺细胞学检查中，苏木精和伊红（H&E）染色或巴氏（Pap）染色的涂片通常比罗曼诺夫斯基染色涂片能更好地评估细胞核特征。然而，H&E染色和Pap染色都需要使用立即湿固定的涂片来保存细胞形态。一定程度的空气干燥通常是不可避免的。发现在95%酒精固定前将空气干燥的涂片置于生理盐水中30秒是一种使细胞再水化的简单方法。如果干燥时间不超过30分钟，再水化涂片的质量优于或等同于立即湿固定涂片的质量。
