Department of Applied Veterinary Science, United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan.
Office of Academic Affairs, Faculty of Veterinary Sciences, Mahasarakham University, Maha Sarakham, Thailand.
Microbiol Immunol. 2020 Oct;64(10):712-718. doi: 10.1111/1348-0421.12835. Epub 2020 Oct 5.
To clarify the persistence of extended-spectrum β-lactamase (ESBL) producers, 13 plasmids from two broiler farms were analyzed. On the farm not using antimicrobials, one plasmid from Klebsiella pneumoniae isolated from a day-old chick was similar to that from Escherichia coli isolated a year later, with the deletion of two transposons. On the farm using antimicrobials, most circulating plasmids (eight out of nine) in a flock of 40-days-old chicks were identical, although one from K. pneumoniae had a deletion of a transposon carrying a class 1 integron containing aadA2 and dfrA12. Thus, ESBL plasmids persisted in the farms with or without antimicrobial agent use.
为了阐明产extended-spectrum β-lactamase (ESBL) 细菌的持续性，我们分析了来自两个肉鸡养殖场的 13 个质粒。在未使用抗生素的养殖场中，从一日龄雏鸡中分离到的肺炎克雷伯菌的一个质粒与一年后从大肠杆菌中分离到的质粒相似，该质粒缺失了两个转座子。在使用抗生素的养殖场中，尽管有一个来自肺炎克雷伯菌的质粒缺失了一个携带包含 aadA2 和 dfrA12 的 class 1 整合子的转座子，但在一群 40 日龄的雏鸡中，大多数循环质粒（9 个中的 8 个）是相同的。因此，无论是否使用抗生素，ESBL 质粒都在养殖场中持续存在。
