Faculty of Applied Biological Science, Gifu University, Gifu, Japan.
Center for Infectious Diseases Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Tokyo, Japan.
J Vet Med Sci. 2023 Jun 1;85(6):613-616. doi: 10.1292/jvms.23-0042. Epub 2023 May 2.
Environmental pollution caused by antimicrobial resistance is a global public health concern. To investigate the contribution of nutrias (Myocastor coypus) to the presence of extended-spectrum β-lactamase (ESBL)-producing Enterobacterales in the Ijira River, prevalence of ESBL-producing Enterobacterales in their feces was examined using deoxycholate-hydrogen sulfide-lactose agar containing cefotaxime. Additionally, the composition of the fecal microbiota of nutria was examined using DNA metabarcoding analysis of the 16S ribosomal RNA gene and compared with that of Amami rabbit, deer, fox, and raccoon dog. The absence of ESBL-producing Enterobacterales and substantially lower abundance of Enterobacterales was observed in the feces of nutrias than in those of other wild mammals. Our results suggest the low potential of antimicrobial-resistant Enterobacterales persistence and dissemination by nutria.
由抗生素耐药性引起的环境污染是一个全球性的公共卫生关注点。为了研究海狸鼠（Myocastor coypus）对伊吉拉河产超广谱β-内酰胺酶（ESBL）的肠杆菌科存在的贡献，使用含有头孢噻肟的去氧胆酸钠-硫化氢-乳糖琼脂检查了它们粪便中 ESBL 产肠杆菌科的流行情况。此外，还使用 16S 核糖体 RNA 基因的 DNA 宏条形码分析检查了海狸鼠粪便中的粪便微生物组组成，并与奄美兔、鹿、狐狸和浣熊犬的粪便微生物组组成进行了比较。与其他野生哺乳动物相比，海狸鼠的粪便中未观察到产 ESBL 的肠杆菌科，且肠杆菌科的丰度也明显较低。我们的研究结果表明，海狸鼠对肠杆菌科具有较低的抗生素耐药性的持久性和传播潜力。
