患者个体癌细胞系和组织分析未提供结直肠癌细胞中存在DNA病毒序列的证据。

Patient-individual cancer cell lines and tissue analysis delivers no evidence of sequences from DNA viruses in colorectal cancer cells.

作者信息

Gock Michael, Kordt Marcel, Matschos Stephanie, Mullins Christina S, Linnebacher Michael

机构信息

Department of General Surgery, University of Rostock, Rostock, Germany.

Department of General Surgery, Molecular Oncology and Immunotherapy, University of Rostock, Schillingallee 35, D-18057, Rostock, Germany.

出版信息

BMC Gastroenterol. 2020 Aug 6;20(1):260. doi: 10.1186/s12876-020-01404-x.

DOI:10.1186/s12876-020-01404-x

PMID:32762707

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7409650/

Abstract

BACKGROUND

Several DNA viruses are highly suspicious to have oncogenic effects in humans. This study investigates the presence of potentially oncogenic viruses such as SV40, JCV, BKV and EBV in patient-derived colorectal carcinoma (CRC) cells typifying all molecular subtypes of CRC.

METHODS

Sample material (gDNA and cDNA) of a total of 49 patient-individual CRC cell lines and corresponding primary material from 11 patients, including normal, tumor-derived and metastasis-derived tissue were analyzed for sequences of SV40, JVC, BKV and EBV using endpoint PCR. In addition, the susceptibility of CRC cells to JCV and BKV was examined using a long-term cultivation approach of patient-individual cells in the presence of viruses.

RESULTS

No virus-specific sequences could be detected in all specimens. Likewise, no morphological changes were observed and no evidence for viral infection or integration could be provided after long term CRC cell cultivation in presence of viral particles.

CONCLUSIONS

In summary, the presented data suggest that there is no direct correlation between tumorigenesis and viral load and consequently no evidence for a functional role of the DNA viruses included into this analysis in CRC development.

摘要

背景

几种DNA病毒极有可能对人类产生致癌作用。本研究调查了患者来源的结肠直肠癌（CRC）细胞中是否存在潜在致癌病毒，如SV40、JCV、BKV和EBV，这些细胞代表了CRC的所有分子亚型。

方法

使用终点PCR分析了总共49个患者个体的CRC细胞系的样本材料（基因组DNA和互补DNA）以及来自11名患者的相应原始材料，包括正常、肿瘤来源和转移来源的组织，以检测SV40、JVC、BKV和EBV的序列。此外，在病毒存在的情况下，采用患者个体细胞长期培养方法，检测CRC细胞对JCV和BKV的敏感性。