Graduate School of Pharmaceutical Sciences, Tokushima University, Tokushima 770-8505, Japan.
School of Pharmacy, Showa University, Shinagawa 142-8555, Japan.
Fitoterapia. 2020 Oct;146:104702. doi: 10.1016/j.fitote.2020.104702. Epub 2020 Aug 5.
Five new abietane diterpenes, lophachinins A-E (1-5), and eleven known related diterpenes were isolated from a Mongolian traditional herbal medicine, the aerial parts of Lophanthus chinensis (Lamiaceae). The structures of new diterpenes were assigned by spectroscopic analyses. Lophachinins A (1) and B (2) were abietane diterpene possessing an endoperoxy bridge at C-ring. In contrast, lophachinins C-E (3-5) had an abietane skeleton with an aromatized C-ring. The absolute configuration of 1 was elucidated by application of the modified Mosher's method, while those of 2, 3, and 5 were assigned by chemical conversions. The absolute configuration of lophachinin D (4) was deduced by ECD calculation. Anti-inflammatory activity of isolated diterpenes on microglial cells were evaluated.
从蒙古族传统草药——垂穗披碱草（Lamiaceae）的地上部分分离得到了 5 个新的扁柏烷二萜类化合物，lophachinins A-E（1-5），和 11 个已知的相关二萜类化合物。通过光谱分析确定了新二萜的结构。lophachinins A（1）和 B（2）是具有 C 环内过氧桥的扁柏烷二萜类化合物。相比之下，lophachinins C-E（3-5）具有芳构化的 C 环的扁柏烷骨架。通过应用改良的 Mosher 法阐明了 1 的绝对构型，而 2、3 和 5 的绝对构型则通过化学转化来确定。通过 ECD 计算推断出 lophachinin D（4）的绝对构型。对分离得到的二萜类化合物在小神经胶质细胞上的抗炎活性进行了评价。
