MRC-Laboratory for Molecular Cell Biology, University College London (UCL), London, UK.
Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden.
Science. 2020 Aug 7;369(6504). doi: 10.1126/science.aaz2532.
is the closest experimentally tractable archaeal relative of eukaryotes and, despite lacking obvious cyclin-dependent kinase and cyclin homologs, has an ordered eukaryote-like cell cycle with distinct phases of DNA replication and division. Here, in exploring the mechanism of cell division in , we identify a role for the archaeal proteasome in regulating the transition from the end of one cell cycle to the beginning of the next. Further, we identify the archaeal ESCRT-III homolog, CdvB, as a key target of the proteasome and show that its degradation triggers division by allowing constriction of the CdvB1:CdvB2 ESCRT-III division ring. These findings offer a minimal mechanism for ESCRT-III-mediated membrane remodeling and point to a conserved role for the proteasome in eukaryotic and archaeal cell cycle control.
是最接近真核生物的实验上可处理的古菌，尽管缺乏明显的细胞周期蛋白依赖性激酶和细胞周期蛋白同源物，但它具有有序的真核样细胞周期，具有明显的 DNA 复制和分裂阶段。在这里，在探索 的细胞分裂机制时，我们发现古菌蛋白酶体在调节从一个细胞周期结束到下一个细胞周期开始的转变中起作用。此外，我们确定了古菌 ESCRT-III 同源物 CdvB 是蛋白酶体的关键靶标，并表明其降解通过允许 CdvB1：CdvB2 ESCRT-III 分裂环的收缩来触发分裂。这些发现为 ESCRT-III 介导的膜重塑提供了一个最小的机制，并指出蛋白酶体在真核生物和古菌细胞周期控制中的保守作用。
