One Health and Veterinary Innovative Research and Development (OHVRI) Group, College of Veterinary Medicine, University of Antioquia, Medellin 050034, Colombia.
Faculty of Agricultural Sciences, School of Zootechny, Universidad de Sucre, Sincelejo 700001, Colombia.
Int J Mol Sci. 2020 Aug 5;21(16):5601. doi: 10.3390/ijms21165601.
l-carnitine is a potent antioxidant used for in vitro culture systems. Controversial results have been reported using l-carnitine in culture medium at different stages of in vitro bovine embryo production. Cumulus-oocyte complexes ( = 843) were in vitro-fertilized and cultured and added (treatment group) or not added (control group) with l-carnitine. At day three of culture, each group was subdivided into two subgroups receiving no l-carnitine (group 1), 3.8 mM l-carnitine added during in vitro maturation (group 2), 1.5 mM added during the in vitro culture (group 3), and 3.8 mM and 1.5 mM added during the maturation and culture, respectively (group 4). At day 8, blastocyst embryos were examined for mitochondrial activity, the presence of lipid droplets, total cell number, gene expression, and cryotolerance by vitrification. The data were analyzed with a one-way analysis of variance. l-carnitine added in the late in vitro culture significantly reduced mitochondrial activity and lipid content, and upregulated and gene expression compared to controls ( < 0.05). l-carnitine supplementation did not significantly affect the embryo rate production or survival rate after vitrification and warming ( > 0.05). l-carnitine supplementation significantly improved embryo potential to develop viable pregnancies in agreement with a study reporting improved pregnancy rates.
左旋肉碱是一种有效的抗氧化剂，用于体外培养系统。在体外牛胚胎生产的不同阶段，在培养基中使用左旋肉碱的结果存在争议。将卵丘-卵母细胞复合物（=843）进行体外受精和培养，并添加（处理组）或不添加（对照组）左旋肉碱。在培养的第 3 天，每组分为两个亚组，不添加左旋肉碱（第 1 组）、体外成熟时添加 3.8 mM 左旋肉碱（第 2 组）、体外培养时添加 1.5 mM 左旋肉碱（第 3 组）以及成熟和培养时分别添加 3.8 mM 和 1.5 mM 左旋肉碱（第 4 组）。在第 8 天，通过玻璃化冷冻对胚胎进行线粒体活性、脂滴存在、总细胞数、基因表达和冷冻耐受性检查。使用单向方差分析对数据进行分析。与对照组相比，晚期体外培养中添加的左旋肉碱显著降低了线粒体活性和脂质含量，并上调了和基因的表达（<0.05）。左旋肉碱补充剂对玻璃化和解冻后的胚胎产率或存活率没有显著影响（>0.05）。左旋肉碱补充剂显著改善了胚胎发育为有活力妊娠的潜力，与一项报道妊娠率提高的研究结果一致。
